Prevalence and Genetic Diversity of Bacillus cereus Isolated from Raw Milk and Cattle Farm Environments

• Published in: Current microbiology Vol. 76; no. 11; pp. 1355 - 1360
• Main Authors: Fei, Peng, Yuan, Xiujuan, Zhao, Shengjuan, Yang, Tongxiang, Xiang, Jinle, Chen, Xi, Zhou, Lianxin, Ji, Mengdi
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2019; Springer Nature B.V
• Subjects: Air Microbiology; Animal Husbandry - instrumentation; Animals; Bacillus cereus; Bacillus cereus - classification; Bacillus cereus - genetics; Bacillus cereus - isolation & purification; Bedding; Biomedical and Life Sciences; Biotechnology; Bovidae; Buckets; Cattle; Cattle - microbiology; Cow's milk; Dairy cattle; Dairy products; Farms; Female; Fodder; Genetic diversity; Genetic Variation; Life Sciences; Livestock farming; Microbiology; Milk; Milk - microbiology; Milking; Milking machines; Multilocus Sequence Typing; Nutrition; Pasteurization; Phylogeny; Shelf life; Soil Microbiology; Soil water; Strains (organisms); Water Microbiology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-019-01741-5

Bacillus cereus not only has adverse effects on the nutrition and shelf life of dairy products but also seriously endanger people’s health. This study was conducted to reveal the prevalence and genetic diversity of B. cereus strains isolated from raw milk and cattle farm environments. A total 56 of B. cereus strains were detected from 300 environmental samples (soil, water, fodder, air, milk pails, milking machines, cowsheds, bedding, excrement, cow surfaces, udders, overalls, soles, and staff hand samples) and 50 raw milk samples, and divided into 18 sequence types (STs) using multilocus sequence typing method. These STs included ST27, ST61, ST92, ST142, ST168, ST208, ST378, ST427, ST766, ST 857, ST1098, ST1140, ST1194, ST1236, ST1336, ST1339, ST1341, and ST1348, among them, ST857 (7/56, 12.5%) was the dominant ST, and were detected from air, cowsheds, bedding, excrement, and raw milk samples. Our findings could reveal the distribution and genetic diversity of B. cereu s strains in raw milk and cattle farm environments, and provide a theoretical basis for controlling the potential harm of this pathogenic bacteria in dairy products.