Enhanced antitumor activity of inulin-capped Se nanoparticles synthesized using Jerusalem artichoke tubers

• Published in: Glycoconjugate journal Vol. 38; no. 5; pp. 599 - 607
• Main Authors: Shao, Taili, Liu, Wei, Yuan, Pingchuan, Li, Xichen, Xu, Honggang, Liu, Chunyan, Chen, Kaoshan, Li, Lihua, Han, Jun, Wang, Guodong
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2021; Springer Nature B.V
• Subjects: Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis; Ascorbic acid; Biochemistry; Biomaterials; Biomedical and Life Sciences; Carcinoma - drug therapy; Cell Line; Glucose tolerance; Helianthus - chemistry; Helianthus tuberosus; Inulin; Inulin - chemistry; Life Sciences; Mice; Molecular weight; Nanoparticles; Nanoparticles - chemistry; Pathology; Plant Tubers - chemistry; Polysaccharides; Selenium - chemistry; Short Communication; Sodium selenite; Stomach Neoplasms; Transmission electron microscopy; Tubers; X-ray spectroscopy; Antitumor activity; Polysaccharides; Se nanoparticles; Jerusalem artichoke; Inulin
• ISSN: 0282-0080; 1573-4986; 1573-4986
• DOI: 10.1007/s10719-021-10011-1

An inulin polysaccharide with a molecular weight of ~ 2600 Da was derived from Jerusalem artichoke tubers and referred to as “JAP”. Previous studies have shown that inulin can improve glucose tolerance and the liver lipid profile; however, its antitumor activity remains to be examined in detail. Therefore, to investigate the possible improvement of the antitumor activity of JAP, a novel nanostructured biomaterial was constructed by capping Se nanoparticles with JAP using sodium selenite, via a redox reaction with ascorbic acid, and referred to as “JAP-SeNPs”. Transmission electron microscopy revealed that the average diameter of JAP-SeNPs is ~ 50 nm, and the C:Se mass ratio in JAP-SeNPs was found to be 15.4:1 by energy-dispersive X-ray spectroscopy. The well-dispersed JAP-SeNPs exhibited a significant in vitro antiproliferative effect on mouse forestomach carcinoma cells at a concentration of 400 μg/mL when incubated for 48 h, with an inhibition rate of 41.5%. Moreover, 38.9% of later apoptotic cells were observed. These results reveal that a combination of Se and JAP can effectively enhance the antitumor activity of polysaccharides obtained from Jerusalem artichoke tubers.