The effects of the neurotoxic agent emamectin benzoate on the expression of immune and stress-related genes and blood serum profiles in the Rainbow trout

• Published in: Molecular biology reports Vol. 47; no. 7; pp. 5243 - 5251
• Main Authors: Kilercioglu, Serdar, Ay, Ozcan, Oksuz, Hale, Yilmaz, Mehmet Bertan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.07.2020; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Antiparasitic Agents - toxicity; Avermectin; Benzoic acid; Biomedical and Life Sciences; Cytokines - genetics; Cytokines - metabolism; Fish Proteins - genetics; Fish Proteins - metabolism; Gene expression; Histology; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Hsp70 protein; IL-1β; Interleukin 8; Ivermectin - analogs & derivatives; Ivermectin - toxicity; Life Sciences; Liver; Liver - drug effects; Liver - metabolism; Morphology; Neurotoxicity; Oncorhynchus mykiss; Original Article; Spleen; Spleen - drug effects; Spleen - metabolism; Stress, Physiological; Trout - blood; Trout - genetics; Trout - metabolism; Tumor necrosis factor-α; Chemokine-IL8; Heat shock protein 70; Serum-biochemical parameters; Proinflammatory cytokines; Rainbow trout; Emamectin benzoate
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-05599-w

Emamectin, a neurotoxic agent, is a semi-synthetic insecticide that belongs to the Avermectin family and is used against helmintic infections in the Salmonidae family. Its secondary effects are not clear; thus, the aim of this study was to investigate the only effects of emamectin benzoate on various biochemical parameters (AST, ALT, GGT, total protein, albumin and glucose) in serum and expressional changes of IL-1β, TNF-α, HSP70 and IL-8 in liver and spleen. For the purpose stated above, rainbow trout (n = 15) were administered 50 μg EB per kg fish daily for 7, 14 and 21 days. The results indicated that weight gains did not change (p  >  0.05), AST increased at day 21 (p < 0.05), while the changes of other biochemical parameters were not significant (p > 0.05). The changes in expression of IL-1β, TNF-α and HSP70 were significant (p < 0.05), while the changes of IL-8 expressions were not significant (p ˃ 0.05). In a conclusion, EB changed immun and stress-related gene expression in liver and spleen, and furthermore, AST changed in a dose- and time-dependent manner. The results imply that emamectin benzoate cause stress. This study is helpful to understand the effects of avermectin pharmaceutical family.