Morphological, fractal, and textural features for the blood cell classification: the case of acute myeloid leukemia

• Published in: European biophysics journal Vol. 50; no. 8; pp. 1111 - 1127
• Main Authors: Dinčić, Marko, Popović, Tamara B., Kojadinović, Milica, Trbovich, Alexander M., Ilić, Andjelija Ž.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.12.2021; Springer Nature B.V
• Subjects: Acute myeloid leukemia; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Blood; Blood Cells; Cell Biology; Classification; Fractal geometry; Fractals; Hematological diseases; Hematology; Homogeneity; Humans; Image Processing, Computer-Assisted; Learning algorithms; Leukemia; Leukemia, Myeloid, Acute; Leukocytes; Life Sciences; Machine learning; Membrane Biology; Morphology; Myeloid leukemia; Nanotechnology; Neurobiology; Nuclei (cytology); Original Article; Parameters; Peripheral blood; Recognition; Cell nucleus architecture; Gray-level co-occurrence matrix (GLCM) features; Fractal dimension; Cell contour; Lacunarity; Cell morphology
• ISSN: 0175-7571; 1432-1017
• DOI: 10.1007/s00249-021-01574-w

Microscopic examination of stained peripheral blood smears is, nowadays, an indispensable tool in the evaluation of patients with hematological and non-hematological diseases. While a rapid automated quantification of the regular blood cells is available, recognition and counting of immature white blood cells (WBC) still relies mostly on the microscopic examination of blood smears by an experienced observer. Recently, there are efforts to improve the prediction by various machine learning approaches. An open dataset collection including the recently digitalized single-cell images for 200 patients, from peripheral blood smears at 100 × magnification, was used. We studied different morphological, fractal, and textural descriptors for WBC classification, with an aim to indicate the most reliable parameters for the recognition of certain cell types. Structural properties of both the mature and non-mature leukocytes obtained from (i) acute myeloid leukemia patients, or (ii) non-malignant controls, were studied in depth, with a sample size of about 25 WBC per group. We quantified structural and textural differences and, based on the statistical ranges of parameters for different WBC types, selected eight features for classification: Cell area , Nucleus-to-cell ratio , Nucleus solidity , Fractal dimension , Correlation , Contrast , Homogeneity , and Energy . Classification Precision of up to 100% (80% on average) was achieved.