Genetic polymorphisms associated with the prevalence of retinal vein occlusion in a Greek population

• Published in: International ophthalmology Vol. 39; no. 11; pp. 2637 - 2648
• Main Authors: Christodoulou, Aikaterini, Bagli, Eleni, Gazouli, Maria, Moschos, Marilita M., Kitsos, Georgios
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.11.2019; Springer Nature B.V
• Subjects: Adiponectin; Aged; Alleles; Anticoagulants; Diabetes mellitus; Eye Proteins - genetics; Eye Proteins - metabolism; Female; Gelatinase A; Gene Frequency; Gene polymorphism; Genetic Predisposition to Disease; Genotype; Genotyping; Glaucoma; Greece - epidemiology; Health risk assessment; Humans; Hyperlipidemia; Hypertension; Male; Medicine; Medicine & Public Health; Occlusion; Ophthalmology; Ophthalmoscopy; Original Paper; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide; Prevalence; Regression analysis; Restriction fragment length polymorphism; Retina; Retinal Vein Occlusion - diagnosis; Retinal Vein Occlusion - epidemiology; Retinal Vein Occlusion - genetics; Retinal Vessels - diagnostic imaging; Risk analysis; Risk Factors; Single-nucleotide polymorphism; Smoking; Greece; VKORC1; AGTR1; RVO; MMP2; Adiponectin; GpIa/IIa
• ISSN: 0165-5701; 1573-2630; 1573-2630
• DOI: 10.1007/s10792-019-01113-9

Purpose To investigate possible associations of single-nucleotide polymorphisms (SNPs) from five genes with branch retinal vein occlusion (BRVO) and central retinal vein occlusion (CRVO). Methods A total of 69 patients with retinal vein occlusion-RVO (24 with BRVO and 45 with CRVO), and 82 controls, were enrolled in this study. All subjects were screened for hypertension, diabetes mellitus, hyperlipidemia, glaucoma, anticoagulant medication, smoking status and history of stroke. The genotyping of AGTR1 -A1166C, adiponectin  + 276 G/T, MMP2 -1306C/T, Gpla/lla -C807T/G873A and VKORC1 -G1639A polymorphisms was performed using restriction fragment length polymorphism or allele-specific polymerase chain reaction. Results The percentage of the AGTR1 -A1166C C allele carriers and Gpla/lla -C807T/G873A T/A carriers was significantly higher in the CRVO patients than in the controls ( P  = 0.00001 and P  = 0.0004, respectively). At the multiple logistic regression analysis, the AGTR1 -A1166C C allele carrier status and the Gpla/lla -C807T/G873A T/A allele carrier status were found to be associated with an increased risk of CRVO. Moreover, adiponectin  + 276 G/T T allele carriers had a significantly increased risk of RVO in subjects ≥ 75 years old. There was no significant difference between the BRVO patients and controls concerning the genotype or the allele frequency distributions of these SNPs. The genotype distributions or allelic frequencies of the other evaluated polymorphisms did not significantly differ between the patients with RVO and the control subjects. Conclusions AGTR1 A1166C and Gpla/lla C807T/G873A polymorphisms are likely to be risk factors for CRVO. Adiponectin  + 276 G/T SNP is likely to predispose to RVO in older subjects.