Paenibacillus arenosi sp. nov., a siderophore-producing bacterium isolated from coastal sediment

• Published in: Archives of microbiology Vol. 204; no. 1; p. 113
• Main Authors: Baek, Jihye, Weerawongwiwat, Veeraya, Kim, Jong-Hwa, Yoon, Jung-Hoon, Lee, Jung-Sook, Sukhoom, Ampaitip, Kim, Wonyong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2022; Springer Nature B.V
• Subjects: Amino acids; Bacteria; Bacterial Typing Techniques; Base Composition; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Ecology; Fatty Acids; Gene clusters; Gene sequencing; Genomes; Hybridization; Life Sciences; Marine sediments; Microbial Ecology; Microbiology; New species; Nucleotides; Original Paper; Paenibacillus; Paenibacillus - genetics; Phospholipids; Phylogenetics; Phylogeny; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Siderophores; Sodium chloride; Sea sand; Whole-genome; Bacillus; Siderophore-producing bacteria; Firmicutes; Paenibacillus arenosi
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02735-3

In this study, strain CAU 1523 T , a novel Gram-positive-positive bacterium isolated from marine sediment collected from the coast of Busan, Republic of Korea, was characterized using a polyphasic taxonomic approach. This strain showed growth at a temperature range of 20–37 °C (optimum, 30 °C), a pH range of 6.5–9.5 (optimum, 7.5), and in the presence of 0–3% (w/v) NaCl (optimum, 1%). Phylogenetic analysis based on 16S rRNA gene sequencing and 92 concatenated core genes indicated that CAU 1523 T belonged to the genus Paenibacillus , sharing the highest sequence similarity with P. assamensis JCM 13186 T (98.0%). CAU 1523 T was differentiated from other Paenibacillus species by average nucleotide identity, average amino acid identity, and digital DNA–DNA hybridization values, using cut-off values of 95–96%, 90%, and 70%, respectively, for closely related strains. The genome of CAU 1523 T possessed various biosynthetic gene clusters, one of which encoded a putative siderophore-interacting protein. Siderophore production by the isolate was confirmed using the qualitative chrome azurol sulfonate (CAS) agar assay. Based on its phylogenetic and physiological characteristics, strain CAU 1523 T represents a novel, siderophore-producing species within the genus Paenibacillus , for which the name Paenibacillus arenosi sp. nov. is proposed, with the type strain CAU 1523 T (= KCTC 43108 T  = MCCC 1K04063 T ).