BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris

• Published in: Chromosoma Vol. 130; no. 2-3; pp. 133 - 147
• Main Authors: do Vale Martins, Lívia, de Oliveira Bustamante, Fernanda, da Silva Oliveira, Ana Rafaela, da Costa, Antônio Félix, de Lima Feitoza, Lidiane, Liang, Qihua, Zhao, Hainan, Benko-Iseppon, Ana Maria, Muñoz-Amatriaín, María, Pedrosa-Harand, Andrea, Jiang, Jiming, Brasileiro-Vidal, Ana Christina
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2021; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Bacterial artificial chromosomes; Biochemistry; Biomedical and Life Sciences; Cell Biology; Chromosome 3; Chromosome rearrangements; Chromosome translocations; Chromosomes; Chromosomes, Plant - genetics; Cytogenetics; Developmental Biology; Divergence; Eukaryotic Microbiology; Evolution; Gene mapping; Human Genetics; Legumes; Life Sciences; Mapping; Original Article; Phaseolus; Phaseolus - genetics; Probes; Species; Synteny; Translocation, Genetic; Vigna; Vigna - genetics; Vigna angularis; Vigna unguiculata; Oligopainting; Beans; Chromosomal rearrangements; BAC-FISH; Macrosynteny; Karyotype evolution
• ISSN: 0009-5915; 1432-0886
• DOI: 10.1007/s00412-021-00758-9

Cytogenomic resources have accelerated synteny and chromosome evolution studies in plant species, including legumes. Here, we established the first cytogenetic map of V. angularis ( Va , subgenus Ceratotropis ) and compared this new map with those of V. unguiculata ( Vu , subgenus Vigna ) and P. vulgaris ( Pv ) by BAC-FISH and oligopainting approaches. We mapped 19 Vu BACs and 35S rDNA probes to the 11 chromosome pairs of Va , Vu , and Pv . Vigna angularis shared a high degree of macrosynteny with Vu and Pv , with five conserved syntenic chromosomes. Additionally, we developed two oligo probes ( Pv 2 and Pv 3) used to paint Vigna orthologous chromosomes. We confirmed two reciprocal translocations (chromosomes 2 and 3 and 1 and 8) that have occurred after the Vigna and Phaseolus divergence (~9.7 Mya). Besides, two inversions (2 and 4) and one translocation (1 and 5) have occurred after Vigna and Ceratotropis subgenera separation (~3.6 Mya). We also observed distinct oligopainting patterns for chromosomes 2 and 3 of Vigna species. Both Vigna species shared similar major rearrangements compared to Pv : one translocation (2 and 3) and one inversion (chromosome 3). The sequence synteny identified additional inversions and/or intrachromosomal translocations involving pericentromeric regions of both orthologous chromosomes. We propose chromosomes 2 and 3 as hotspots for chromosomal rearrangements and de novo centromere formation within and between Vigna and Phaseolus . Our BAC- and oligo-FISH mapping contributed to physically trace the chromosome evolution of Vigna and Phaseolus and its application in further studies of both genera.