Gut Lactobacillus and Probiotics Lactobacillus lactis/rhamnosis Ameliorate Liver Fibrosis in Prevention and Treatment

• Published in: The journal of microbiology Vol. 61; no. 2; pp. 245 - 257
• Main Authors: Won, Sung Min, Lee, Na Young, Oh, Ki-Kwang, Gupta, Haripriya, Sharma, Satya Priya, Kim, Kyung Hwan, Kim, Byoung Kook, Joung, Hyun Chae, Jeong, Jin Ju, Ganesan, Raja, Han, Sang Hak, Yoon, Sang Jun, Kim, Dong Joon, Suk, Ki Tae
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.02.2023; Springer Nature B.V
• Subjects: Animal models; Animals; Biomedical and Life Sciences; Carbon tetrachloride; Cecum; Cirrhosis; Digestive system; Evaluation; Fibrosis; Gene expression; Humans; Intestinal flora; Intestinal microflora; Lacticaseibacillus rhamnosus; Lactobacillus; Life Sciences; Liver; Liver cirrhosis; Liver Cirrhosis - prevention & control; Mice; Mice, Inbred C57BL; Microbial Pathogenesis and Host-Microbe Interaction; Microbiology; Microbiomes; Microbiota; Microorganisms; Prevention; Probiotics; Serum; Probiotics; Gut microbiome; Liver fibrosis; Lactobacillus lactis; Lactobacillus rhamnosus
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-023-00014-y

The progression and exacerbation of liver fibrosis are closely related to the gut microbiome. It is hypothesized that some probiotics may slow the progression of liver fibrosis. In human stool analysis [healthy group ( n  = 44) and cirrhosis group ( n  = 18)], difference in Lactobacillus genus between healthy group and cirrhosis group was observed. Based on human data, preventive and therapeutic effect of probiotics Lactobacillu s lactis and L. rhamnosus was evaluated by using four mice fibrosis models. L. lactis and L. rhamnosus were supplied to 3,5-diethoxycarbonyl-1,4-dihydrocollidine or carbon tetrachloride-induced liver fibrosis C57BL/6 mouse model. Serum biochemical measurements, tissue staining, and mRNA expression in the liver were evaluated. The microbiome was analyzed in mouse cecal contents. In the mouse model, the effects of Lactobacillus in preventing and treating liver fibrosis were different for each microbe species. In case of L. lactis , all models showed preventive and therapeutic effects against liver fibrosis. In microbiome analysis in mouse models administered Lactobacillus , migration and changes in the ratio and composition of the gut microbial community were confirmed. L. lactis and L. rhamnosus showed preventive and therapeutic effects on the progression of liver fibrosis, suggesting that Lactobacillus intake may be a useful strategy for prevention and treatment.