Cervicovaginal microbiota disorder combined with the change of cytosine phosphate guanine motif- toll like receptor 9 axis was associated with cervical cancerization

• Published in: Journal of cancer research and clinical oncology Vol. 149; no. 19; pp. 17371 - 17381
• Main Authors: Zheng, Xiao, Hu, Nan, Liu, Jiamin, Zhao, Kailu, Li, Huimin, Wang, Jiahao, Zhang, Mingxuan, Zhang, Le, Song, Li, Lyu, Yuanjing, Cui, Meng, Ding, Ling, Wang, Jintao
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2023; Springer Nature B.V
• Subjects: Bacteria; Cancer Research; Carcinogenesis; Cervical cancer; Cervix Uteri - microbiology; CpG islands; Cytosine; Dysbacteriosis; Female; Hematology; Humans; Internal Medicine; Medicine; Medicine & Public Health; Metagenomics; Microbiota; Oncology; Phosphates; TLR9 protein; Toll-Like Receptor 9 - genetics; Toll-Like Receptor 9 - metabolism; Toll-like receptors; Uterine Cervical Dysplasia - genetics; Uterine Cervical Dysplasia - microbiology; Uterine Cervical Dysplasia - pathology; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - microbiology; Uterine Cervical Neoplasms - pathology; Vagina - microbiology; Western blotting; Cervicovaginal microbiota; TLR9; Cervical lesion; Metagenomic shotgun sequencing; CpG motif
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-023-05453-z

Background Convincing studies demonstrated that cervicovaginal microbiota disorder and toll-like receptor 9 (TLR9) high expression were related to cervical carcinogenesis. However, the effects of cervicovaginal microbiota integration TLR9 in cervical cancerization are unclear. Based on the biological basis that unmethylated cytosine–phosphate–guanine (CpG) motifs of bacteria could activate TLR9, we explored the effects of cervicovaginal microbiota disorder and CpG motif–TLR9 axis change in cervical carcinogenesis. Methods A total of 341 participants, including 124 normal cervical (NC), 90 low-grade cervical intraepithelial neoplasia (CIN1), 78 high-grade cervical intraepithelial neoplasia (CIN2/3) and 49 squamous cervical cancer (SCC), diagnosed by pathology were enrolled in the study. Here, metagenomic shotgun sequencing was used to reveal cervicovaginal microbiota characteristics, and TLR9 protein was detected by western blotting. Results Our results showed that the diversity of cervicovaginal microbiota gradually increased along with the poor development of cervical lesions, showing the abundance of Lactobacillus crispatus and Lactobacillus iners decreased, while the abundance of pathogenic bacteria gradually increased. The level of TLR9 expression was gradually increased with cervicovaginal microbiota diversity increasing, the abundance of Lactobacillus decreasing, and we found a positive correlation dependency relationship ( r  = 0.384, P  = 0.002) between TLR9 and GTCGTT motif content. Stratified analysis based on HPV16 infection, we found that the characteristics of cervicovaginal microbiota and increased TLR9 expression were also closely related to HPV16 infection. Conclusions Cervicovaginal microbiota dysbiosis might lead to the CpG motif increased, which was closely associated with TLR9 high expression, and ultimately might promote the progression of cervical lesions.