MicroRNA-155-IFN-γ Feedback Loop in CD4(+)T Cells of Erosive type Oral Lichen Planus

• Published in: Scientific reports Vol. 5; no. 1; p. 16935
• Main Authors: Hu, Jing-Yu, Zhang, Jing, Ma, Jing-Zhi, Liang, Xue-Yi, Chen, Guan-Ying, Lu, Rui, Du, Ge-Fei, Zhou, Gang
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 23.11.2015
• Subjects: Adult; Case-Control Studies; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - pathology; Cell Differentiation; Cell Proliferation; Feedback, Physiological; Female; Gene Expression Regulation; Humans; Interferon-gamma - genetics; Interferon-gamma - immunology; Interferon-gamma - pharmacology; Interleukin-10 - genetics; Interleukin-10 - immunology; Interleukin-2 - genetics; Interleukin-2 - immunology; Lichen Planus, Oral - classification; Lichen Planus, Oral - genetics; Lichen Planus, Oral - immunology; Lichen Planus, Oral - pathology; Male; MicroRNAs - genetics; MicroRNAs - immunology; Middle Aged; Primary Cell Culture; RNA, Messenger - genetics; RNA, Messenger - immunology; Severity of Illness Index; Signal Transduction; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - immunology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep16935

Oral lichen planus (OLP) is a T cell-mediated immune disorder, and we have indicated a Th1-dominated immune response in OLP. MicroRNA-155 (miR-155) could promote Th1 cells polarization. The present study aims to determine the role of miR-155 in immune response of OLP. The expression of miR-155 and the target mRNA was tested by Real-Time PCR. The serum levels of IL-2, 4, 10 and IFN-γ were examined with ELISA. Furthermore, in vitro study was built to observe the function of miR-155 in erosive-type OLP (EOLP). Finally, we determined the expression and correlation of miR-155 and SOCS1 in EOLP CD4(+) T cells. The results showed miR-155 was high related with the disease severities. Besides, serum IFN-γ was specifically increased in EOLP group, while IL-4 was decreased. In vitro studies showed miR-155 could reinforce IFN-γ signal transducer, and the induction of IFN-γ could also promote miR-155 expression in EOLP CD4(+) T cells. In addition, miR-155 levels were negatively related with SOCS1 mRNA expression in EOLP CD4(+) T cells. Our study revealed a positive miR-155- IFN-γ feedback loop in EOLP CD4(+) T cell, which might contribute to the Th1-dominated immune response. Furthermore, miR-155 could be used for the evaluation and treatment of OLP.