Investigation of drought induced biochemical and gene expression changes in carrot cultivars

• Published in: Molecular biology reports Vol. 50; no. 1; pp. 349 - 359
• Main Authors: Junaid, Muhammad Daniyal, Öztürk Gökçe, Zahide Neslihan, Gökçe, Ali Fuat
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2023; Springer Nature B.V
• Subjects: Actin; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Carrots; Catalase; Cultivars; Daucus carota - genetics; Daucus carota - metabolism; Drought resistance; Droughts; Enzymatic activity; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Plant - genetics; Histology; Life Sciences; Morphology; Nutrient deficiency; Original Article; Plant Breeding; Proline dehydrogenase; Proline transporter; Stress, Physiological - genetics; Superoxide dismutase; Vegetables; Proline transporters; Drought stress; Superoxide dismutase; L; Biochemical analyses; Proline dehydrogenase; Daucus carota L
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-022-08050-4

Background Carrot is the most important vegetable in Apiaceae family, and it is consumed globally due to its high nutritional quality. Drought stress is major environmental constraint for vegetables especially carrot. Limited data is available regarding the mechanisms conferring drought tolerance in carrot. Methods and Results Eight commercial carrot cultivars were used in this study and subjected to drought stress under semi-controlled greenhouse conditions. Biochemical, antioxidant enzymatic activity and changes in transcript level of drought related genes was estimated, the gene expression analysis was done by using qRT-PCR in comparison with reference gene expression Actin ( Act1 ). Results revealed that cultivars Coral Orange, Tendersweet and Solar Yellow were tolerant to drought stress, which was supported by their higher transcript levels of catalase gene ( CAT ), superoxide dismutase genes ( Cu/ZN-SOD, Cu/Zn-SDC ) in these cultivars. The downregulation of PDH1 gene (Proline dehydrogenase 1) was also observed that was associated with upregulation of proline accumulation in carrot plants. Moreover, results also suggested that PRT genes (Proline transporter genes) played a key role in drought tolerance in carrot cultivars. Conclusion Among the cultivars studied, Coral Orange showed overall tolerance to drought stress conditions, whereas cultivars Cosmic Purple and Eregli Black were sensitive based on their biochemical and gene expression levels. According to our knowledge, this is the first comparative study on drought tolerance in several carrot cultivars. It will provide a background for carrot breeding to understand biochemical and molecular responses of carrot plant to drought stress and mechanisms behind it.