Cytotoxicity screening of supercritical fluid extracted seaweeds and phenylpropanoids

• Published in: Molecular biology reports Vol. 46; no. 4; pp. 3691 - 3699
• Main Authors: Sevimli-Gur, Canan, Yesil-Celiktas, Ozlem
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2019; Springer Nature B.V
• Subjects: Adenocarcinoma; Alismatales - chemistry; Animal Anatomy; Animal Biochemistry; Animals; Antineoplastic Agents - pharmacology; Benzoic Acid - pharmacology; Biomedical and Life Sciences; Caffeic Acids - pharmacology; Carbon dioxide; Cervical cancer; Chlorocebus aethiops; Cinnamates - pharmacology; Colon; Colon cancer; Coumaric acid; Coumaric Acids - pharmacology; Cytotoxicity; Depsides - pharmacology; HeLa Cells; Histology; Humans; Life Sciences; Mass Screening; MCF-7 Cells; Morphology; Neuroblastoma; Original Article; p-Coumaric acid; PC-3 Cells; Phenylpropanoids; Plant Extracts - pharmacology; Posidonia oceanica; Propionates - metabolism; Propionates - pharmacology; Prostate; Rosmarinic Acid; Seaweed - chemistry; Seaweeds; Shores; Solvents; Succinates - pharmacology; Tumor cell lines; Vero Cells; Zostera marina; Zosteraceae - chemistry; Cytotoxicity; Supercritical fluid; Green extraction technologies; Seaweed waste; Phenylpropanoid; Zostera marina; Posidonia oceanica
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-019-04812-9

Detached leaves of Posidonia oceanica and Zostera marina creating nuisance at the shores were extracted by means of supercritical CO 2 enriched with a co-solvent, compared with that of soxhlet extraction. The extracts and their active compounds which are phenylpropanoids (chicoric, p-coumaric, rosmarinic, benzoic, ferulic and caffeic acids) were screened for cytotoxicity in cancer cell lines including human breast adenocarcinoma (MCF-7, MDA-MB-231, SK-BR-3), human colon adenocarcinoma (HT-29), human cervix adenocarcinoma (HeLa), human prostate adenocarcinoma (PC-3), Mus musculus neuroblastoma (Neuro 2A) cell lines and African green monkey kidney (VERO) as healthy cell line. Supercritical CO 2 extracts proved to be more active than soxhlet counterparts. Particularly, Zostera marina extract obtained by supercritical CO 2 at 250 bar, 80 °C, 20% co-solvent and a total flow rate of 15 g/min revealed the best IC 50 values of 25, 20, 8 μg/ml in neuroblastoma, colon and cervix cancer cell lines. Among the major compounds tested, p-coumaric acid exhibited the highest cytotoxic against colon and cervix cell lines by with IC 50 values of 25, 11 μg/ml. As for the effects on healthy cells, the extract was not cytotoxic indicating a selective cytotoxicity. Obtained supercritical CO 2 extracts can be utilized as a supplement for preventive purposes.