Relationship between heat treatment on structural properties and antitumor activity of the cold-water soluble polysaccharides from Grifola frondosa

• Published in: Glycoconjugate journal Vol. 37; no. 1; pp. 107 - 117
• Main Authors: Li, Xiao-Qing, Liu, An-Jun
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2020; Springer Nature B.V
• Subjects: Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor activity; Biochemistry; Biodegradation; Biomedical and Life Sciences; Carbohydrate Conformation; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Chromatography; Food industry; Fourier transforms; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - pharmacology; Fungi; Gas chromatography; Grifola - chemistry; Heat; Hot Temperature; Humans; Infrared spectroscopy; Life Sciences; Molecular weight; NMR; Nuclear magnetic resonance; Original Article; Oxidation; Oxidation-Reduction; Pathology; Polysaccharides; Structure-Activity Relationship; Structure-activity relationships; polysaccharides; Heat treatment; Structural characteristics; Antitumor activities; Grifola frondosa polysaccharides
• ISSN: 0282-0080; 1573-4986; 1573-4986
• DOI: 10.1007/s10719-019-09894-y

Grifola frondosa is a basidiomycete fungus with potential biomedical applications owing to the presence of bioactive polysaccharides. The activities of polysaccharides are influenced by many factors, particularly temperature; however, the optimal temperature and conditions for preparation of polysaccharides from this organism have not yet been determined. Therefore, in this study, cold-water soluble polysaccharides from Grifola frondosa were extracted at 4 °C (GFP-4) and purified. GFP-4-30, GFP-4-60 and GFP-4-90 were obtained from GFP-4 after treatment at 30 °C, 60 °C, or 90 °C, respectively, for 6 h. MTT results showed that GFP-4 had the highest inhibitory effects on the proliferation of SPC-A-1 cells in vitro . High-performance gel permeation chromatography results demonstrated that the molecular weight of GFP-4 was 1.05 × 10 6 Da and that GFP-4-30, GFP-4-60, and GFP-4-90 showed different levels of degradation and generated small molecule sugars. Fourier transform infrared spectroscopy, gas chromatography, and nuclear magnetic resonance results indicated that GFPs mainly consisted of α- d -Gal p , α- d -Man p and α- d -Glc p . Periodate oxidation, Smith degradation, and methylation results showed that the backbones of the molecules consisted of 1,3-linked-Gal p . After heat treatment, percentages of (1 → 3,4) α- d -Gal p in heat-treated polysaccharides were obviously decreased, indicating their lower branching degree, and resulting in weaker antitumor effects. Overall, our findings demonstrated changes in the structure-activity relationships of GFP-4 after heat treatment and provided a theoretical basis for the application of GFP-4 in the food and drug industries.