Insights into the binding mechanism of ascorbic acid and violaxanthin with violaxanthin de-epoxidase (VDE) and chlorophycean violaxanthin de-epoxidase (CVDE) enzymes

• Published in: Photosynthesis research Vol. 156; no. 3; pp. 337 - 354
• Main Authors: Biswal, Satyaranjan, Gupta, Parth Sarthi Sen, Panda, Saroj Kumar, Bhat, Haamid Rasool, Rana, Malay Kumar
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2023; Springer Nature B.V
• Subjects: Acids; Algae; Ascorbic acid; Biochemistry; Biomedical and Life Sciences; Conformation; Enzymes; Epoxidation; Homology; Hydrogen bonding; Life Sciences; Oxidoreductases - metabolism; Phylogeny; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Reactive oxygen species; Thylakoid membranes; Thylakoids - metabolism; Violaxanthin; Violaxanthin de-epoxidase; Xanthophylls; Xanthophylls - metabolism; Molecular dynamics; DFT; CVDE; Xanthophyll cycle; VDE; Free energy
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-023-01006-0

Photosynthetic organisms have evolved to work under low and high lights in photoprotection, acting as a scavenger of reactive oxygen species. The light-dependent xanthophyll cycle involved in this process is performed by a key enzyme (present in the thylakoid lumen), Violaxanthin De-Epoxidase (VDE), in the presence of violaxanthin (Vio) and ascorbic acid substrates. Phylogenetically, VDE is found to be connected with an ancestral enzyme Chlorophycean Violaxanthin De-Epoxidase (CVDE), present in the green algae on the stromal side of the thylakoid membrane. However, the structure and functions of CVDE were not known. In search of functional similarities involving this cycle, the structure, binding conformation, stability, and interaction mechanism of CVDE are explored with the two substrates compared to VDE. The structure of CVDE was determined by homology modeling and validated. In silico docking (of first-principles optimized substrates) revealed it has a larger catalytic domain than VDE. A thorough analysis of the binding affinity and stability of four enzyme–substrate complexes is performed by computing free energies and their decomposition, the root-mean-square deviation (RMSD) and fluctuation (RMSF), the radius of gyration, salt bridge, and hydrogen bonding interactions in molecular dynamics. Based on these, violaxanthin interacts with CVDE to a similar extent as that of VDE. Hence, its role is expected to be the same for both enzymes. On the contrary, ascorbic acid has a weaker interaction with CVDE than VDE. Given these interactions drive epoxidation or de-epoxidation in the xanthophyll cycle, it immediately discerns that either ascorbic acid does not participate in de-epoxidation or a different cofactor is necessary as CVDE has a weaker interaction with ascorbic acid than VDE. Graphical abstract