ZmMYB31, a R2R3-MYB transcription factor in maize, positively regulates the expression of CBF genes and enhances resistance to chilling and oxidative stress

• Published in: Molecular biology reports Vol. 46; no. 4; pp. 3937 - 3944
• Main Authors: Li, Meng, Lin, Lin, Zhang, Yuanhu, Sui, Na
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2019; Springer Nature B.V
• Subjects: Adaptation, Physiological - genetics; Animal Anatomy; Animal Biochemistry; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Ascorbic acid; Biomedical and Life Sciences; CBF protein; Chilling; Cold Temperature - adverse effects; Cold-Shock Response - genetics; Extravasation; Gene Expression Regulation, Plant - genetics; Genes, Plant; Histology; L-Ascorbate peroxidase; Life Sciences; Morphology; Original Article; Oxidation-Reduction; Oxidative stress; Oxidative Stress - physiology; Peroxidase; Peroxide; Photoinhibition; Photosystem II; Plant Proteins - genetics; Plants, Genetically Modified - genetics; Reaction centers; Reactive oxygen species; Reactive Oxygen Species - metabolism; Stress, Physiological - genetics; Superoxide dismutase; Temperature; Temperature effects; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transgenic plants; Zea mays; Zea mays - genetics; Zea mays - metabolism; Chilling stress; Oxidative stress; Maize; ZmMYB31
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-019-04840-5

Maize ( Zea mays L.) is an important model plant with an important role in agriculture and national economies all over the world. The optimum growth temperature of maize is between 25 and 28 °C. At temperatures below 12 °C, maize is vulnerable to damage by chilling stress. MYB transcription factors play important roles in plants’ response to low temperature stress. Maize ZmMYB31 encodes a R2R3-MYB transcription factor, ZmMYB31, which localized in the nucleus. ZmMYB31 expression was induced by chilling stress and the highest expression level was detected with the 24 h chilling treatment. ZmMYB31 expression also increased in overexpressing Arabidopsis lines. The minimal fluorescence (Fo) with all photosystem II reaction centers open increased in wild type (WT) and transgenic plants under chilling stress, with the highest increase in WT. The maximal photochemical efficiency of photosystem II (Fv/Fm) decreased more in WT than in transgenic plants during chilling stress. Furthermore, the ZmMYB31 -overexpressing lines showed higher superoxide dismutase and ascorbate peroxidase activity and lower reactive oxygen species (ROS) content than the WT. The expression of genes related to chilling stress was higher in transgenic plants than in WT. These results suggest that ZmMYB31 plays a positive regulatory role in chilling and peroxide stress by regulating the expression of chilling stress-related genes to reduce ion extravasation, ROS content, and low-temperature photoinhibition, thereby improving low temperature resistance.