Secretome analysis identified extracellular superoxide dismutase and catalase of Macrophomina phaseolina
Macrophomina phaseolina , a necrotrophic fungal pathogen is known to cause charcoal rot disease in food crops, pulse crops, oil crops and cotton and fibre crops. Necrotrophic fungi survive on dead plant tissue. It is well known that reactive oxygen species (ROS) are produced by the host plant during...
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Published in | Archives of microbiology Vol. 204; no. 1; p. 62 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.01.2022
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Macrophomina phaseolina
, a necrotrophic fungal pathogen is known to cause charcoal rot disease in food crops, pulse crops, oil crops and cotton and fibre crops. Necrotrophic fungi survive on dead plant tissue. It is well known that reactive oxygen species (ROS) are produced by the host plant during plant–pathogen interaction. However, it is still unclear how
M. phaseolina
can overcome the ROS-induced cellular damage. To mimic the invasion of
M. phaseolina
inside the plant cell wall, we developed solid substrate fermentation where
M. phaseolina
spore suspension was inoculated on a wheat bran bed and incubated for vegetative growth. To analyse the secretome of
M. phaseolina
after different day interval, its secretory material was collected and concentrated. Both superoxide dismutase (SOD) and catalase were detected in the secretome by zymogram. The presence of SOD and catalase was further confirmed by liquid chromatography based mass spectrometry. The physicochemical properties of
M. phaseolina
catalase in terms of stability towards pH, temperature, metal ions and chaotropic agent and inhibitors indicated its fitness at different environmental conditions. Apart from the production of catalase in SSF, the studies on this particular microorganism may also have significance in necrotrophic fungal pathogen and their susceptible host plant interaction. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0302-8933 1432-072X |
DOI: | 10.1007/s00203-021-02631-w |