Secretome analysis identified extracellular superoxide dismutase and catalase of Macrophomina phaseolina

• Published in: Archives of microbiology Vol. 204; no. 1; p. 62
• Main Authors: Sinha, Nilanjan, Patra, Sourav Kumar, Sarkar, Tuhin Subhra, Ghosh, Sanjay
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2022; Springer Nature B.V
• Subjects: Ascomycota - enzymology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Catalase; Cell Biology; Cell walls; Charcoal rot; Cotton; Crops; Ecology; Environmental conditions; Fermentation; Fungi; Herbivores; Host plants; Life Sciences; Liquid chromatography; Macrophomina phaseolina; Mass spectrometry; Mass spectroscopy; Metal ions; Microbial Ecology; Microbiology; Oilseeds; Original Paper; Pathogens; Physicochemical properties; Plant Diseases - microbiology; Reactive oxygen species; Secretome; Substrates; Superoxide Dismutase; Wheat bran; Fungal secretome; Extracellular superoxide dismutase; Solid-state fermentation; Extracellular catalase; Necrotrophic fungus; Macrophomina phaseolina
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02631-w

Macrophomina phaseolina , a necrotrophic fungal pathogen is known to cause charcoal rot disease in food crops, pulse crops, oil crops and cotton and fibre crops. Necrotrophic fungi survive on dead plant tissue. It is well known that reactive oxygen species (ROS) are produced by the host plant during plant–pathogen interaction. However, it is still unclear how M. phaseolina can overcome the ROS-induced cellular damage. To mimic the invasion of M. phaseolina inside the plant cell wall, we developed solid substrate fermentation where M. phaseolina spore suspension was inoculated on a wheat bran bed and incubated for vegetative growth. To analyse the secretome of M. phaseolina after different day interval, its secretory material was collected and concentrated. Both superoxide dismutase (SOD) and catalase were detected in the secretome by zymogram. The presence of SOD and catalase was further confirmed by liquid chromatography based mass spectrometry. The physicochemical properties of M. phaseolina catalase in terms of stability towards pH, temperature, metal ions and chaotropic agent and inhibitors indicated its fitness at different environmental conditions. Apart from the production of catalase in SSF, the studies on this particular microorganism may also have significance in necrotrophic fungal pathogen and their susceptible host plant interaction.