Flagellimonas hymeniacidonis sp. nov., Isolated from the Sponge Hymeniacidon sinapium

• Published in: Current microbiology Vol. 78; no. 3; pp. 1061 - 1067
• Main Authors: Shin, Tae-Gi, Park, Jin-Sook
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2021; Springer Nature B.V
• Subjects: Animals; Bacterial Typing Techniques; Biomedical and Life Sciences; Biotechnology; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty Acids; Flavobacteriaceae - genetics; Gene sequencing; Gram stain; Hybridization; Hymeniacidon sinapium; Isoprene; Life Sciences; Lipids; Menaquinones; Microbiology; New species; Nucleotides; pH effects; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Quinones; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Vitamin K 2; Republic of Korea
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-020-02328-1

A Gram-stain-negative, rod-shaped, non-motile, aerobic, yellow-pigmented, marine bacterium (designated strain 176CP5-101  T ) was isolated from a sponge Hymeniacidon sinapium from Cheongpo beach, Taean in Republic of Korea. The strain was able to grow at pH 6.8–8.5 (optimum at pH 7.5), in 1–7% (w/v) NaCl (optimum at 3%, w/v) and at 10–30 °C (optimum at 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 176CP5-101  T belonged to the family Flavobacteriaceae and was most closely related to Flagellimonas aquimarina KCTC 52351  T (97.1%), F . algicola KACC 19790  T (97.0%), F . pacifica KCTC 52762  T (96.9%), F . eckloniae KCCM 42307  T (96.4%), F . flava KCTC 22665  T (96.0%), and F. maritima KCTC 32464  T (95.4%). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain 176CP5-101  T and F . aquimarina KCTC 52351  T were, respectively, 79.9% and 22.1%, indicating that 176CP5-101  T is a novel species of the genus Flagellimonas . The DNA G + C content of the genomic DNA was 37.8 mol%. Menaquinone with six isoprene units (MK-6) was the major respiratory quinone, and the major polar lipids were phosphatidylethanolamine (PE), unidentified aminophospholipid, and unidentified lipids. The fatty acids are mainly (> 5%) defined iso-C 15:0 , iso-C 15:0 3-OH, iso-C 15:1 G, iso-C 17:0 3-OH, and C 16:1 ω 7 c and/or C 16:1 ω 6 c (summed feature 3). Phenotypic, phylogenetic, genomic, and chemotaxonomic characteristics showed that strain 176CP5-101  T represents a novel species of the genus Flagellimonas , for which the name Flagellimonas hymeniacidonis sp. nov. is proposed. The type strain is 176CP5-101  T (= KACC 19892  T  = LMG 31316  T ).