TEAD4 overexpression suppresses thyroid cancer progression and metastasis in vitro by modulating Wnt signaling

• Published in: Journal of biosciences Vol. 47; no. 1
• Main Authors: Zhang, Buyong, Wang, Qingqing, Ji, Yanting, Zhang, Xuan, Xue, Lingbo, Shi, Qingfeng, Li, Jie
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.03.2022; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Cancer; Cell Biology; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell Proliferation - genetics; Cell viability; Cells; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; E-cadherin; Epithelial-Mesenchymal Transition - genetics; Gene Expression Regulation, Neoplastic; Humans; Kinases; Life Sciences; Metastases; Metastasis; Microbiology; Muscle Proteins - genetics; Muscle Proteins - metabolism; N-Cadherin; Nucleotide sequence; PCR; Plant Sciences; Plasmids; Regulatory mechanisms (biology); Signaling; TEA Domain Transcription Factors; Thyroid; Thyroid cancer; Thyroid gland; Thyroid Neoplasms - genetics; Thyroid Neoplasms - pathology; Tissue; Transcription Factors - genetics; Transcription Factors - metabolism; Vimentin; Wnt protein; Wnt Signaling Pathway - genetics; Zoology; TEAD4; Epithelial-Mesenchymal transition; Wnt signaling; Thyroid cancer; Migration/invasion
• ISSN: 0250-5991; 0973-7138
• DOI: 10.1007/s12038-021-00238-3

TEAD4 has been reported to act as an oncogenic gene in various types of cancer. This study intends to investigate the role and regulatory mechanism of TEAD4 in thyroid cancer (TC). GEPIA was used to predict the expression pattern of TEAD4 in TC. Expressions of TEAD4 and wnt3a in TC tissues and cells were analyzed by qRT-PCR and Western blot. TC cells were transfected with TEAD4 overexpression plasmids and treated with or without IWR-1-endo (a Wnt signaling inhibitor), and then TC cell viability, migration and invasion were assessed by MTT and Transwell assay. Expressions of E-cadherin, N-cadherin and Vimentin in cells were analyzed by Western blot. TEAD4 was low-expressed in TC tissues and cells. TEAD4 overexpression inhibited the viability, inhibited migration and invasion of TC cells, and downregulated N-cadherin and Vimentin expression, while promoted E-cadherin and wnt3a expression. The wnt3a expression was positively correlated with TEAD4 expression in TC. IWR-1-endo treatment reversed the effect of TEAD4 in TC cells. TEAD4 overexpression suppresses TC progression and metastasis in vitro through modulating Wnt signaling.