Lacticaseibacillus casei LH23 Suppressed HPV Gene Expression and Inhibited Cervical Cancer Cells

• Published in: Probiotics and antimicrobial proteins Vol. 15; no. 3; pp. 443 - 450
• Main Authors: Hu, Shiyue, Hao, Yunpeng, Zhang, Xiao, Yang, Yaping, Liu, Meiling, Wang, Nan, Zhang, Tong-Cun, He, Hongpeng
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2023; Springer Nature B.V
• Subjects: Animals; Apoptosis; Applied Microbiology; Biomedical and Life Sciences; Cell migration; Cell proliferation; Cervical cancer; Cervix; Chemistry/Food Science; Colitis; Female; Fermented food; Gene Expression; Human papillomavirus; Humans; Lacticaseibacillus; Lacticaseibacillus casei; Lactobacillus; Life Sciences; Lysates; Metastases; Mice; Microbiology; Nutrition; Papillomavirus E7 Proteins - genetics; Papillomavirus Infections; Protein Science; Reproductive system; Uterine Cervical Neoplasms - drug therapy; Wound healing; Proliferation; Migration; Cervical cancer; HPV; Apoptosis; Lactobacillus
• ISSN: 1867-1306; 1867-1314
• DOI: 10.1007/s12602-021-09848-7

Microbiota of lower female reproductive tract is special in its microorganism composition with Lactobacillus as the predominant bacteria. A few of Lactobacillus species have been identified to benefit the inhibition of inflammatory and malignant diseases. Lacticaseibacillus casei LH23 is a strain isolated from traditional fermented food and had been demonstrate to ameliorate DSS-induced colitis in mice. In the present study, effects of Lacticaseibacillus casei LH23 on cervical cancer cells were investigated. Supernatants of lysates and heat-inactivated Lacticaseibacillus casei LH23 were found to inhibit the expression of human papillomavirus genes E6/E7 which is the main causative factor of cervical cancer. With MTT, EdU staining, and TUNEL staining assays, Lacticaseibacillus casei LH23 was shown to suppress the proliferation and induced the apoptosis of cervical cancer cells. Additionally, with wound-healing and Western-blot assays, Lacticaseibacillus casei LH23 was shown to slowdown the migration of cervical cancer cells and altered the expression of metastasis-related genes. These results demonstrated the anti-cervical cancer potential of Lacticaseibacillus casei LH23.