Isolation and complete genome sequencing of the virulent phage vB_EcoS_XY3 infecting multidrug-resistant Escherichia coli

• Published in: Archives of virology Vol. 166; no. 1; pp. 303 - 307
• Main Authors: Fu, Pan, Zhao, Qiang, Shi, Li, Xiong, Qin, Xu, Hongxia, Cai, Senmao, Xu, Qianqian, Sun, Xiaodong, Sang, Ming
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 2021; Springer Nature B.V
• Subjects: Annotated Sequence Record; Bacteriophages - genetics; Base Composition - genetics; Biological control; Biomedical and Life Sciences; Biomedicine; China; DNA - genetics; DNA, Viral - genetics; Drug Resistance, Multiple, Bacterial - genetics; E coli; Escherichia coli; Escherichia coli - virology; Genome, Viral - genetics; Genomes; Infectious Diseases; Medical Microbiology; Medical wastes; Multidrug resistance; Multidrug resistant organisms; Phages; Physical characteristics; Sequence Analysis, DNA - methods; Virology; Virulence - genetics; Waste Water - virology; Wastewater; Whole Genome Sequencing - methods; China
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-020-04844-8

A virulent phage, named vB_EcoS_XY3, was isolated from hospital wastewater in Xiangyang, China. Its morphological characteristics, growth parameters, adsorption rate, and pH and temperature stability were determined. Phage vB_EcoS_XY3 was found to be able to infect Escherichia coli laboratory strains and also some multidrug-resistant E. coli strains. Its complete genome consists of 51,345 base pairs of double-stranded DNA with an average GC content of 55.24% and 85 putative protein-coding genes. Forty-four genes were annotated with known functions. These results will not only provide further insights into E. coli phages but also have implications for the development of potential biocontrol agents.