Simultaneous colonization by Escherichia coli and Klebsiella pneumoniae harboring mcr-1 in Brazil

• Published in: Infection Vol. 47; no. 4; pp. 661 - 664
• Main Authors: Perdigão Neto, Lauro Vieira, Corscadden, Louise, Martins, Roberta Cristina Ruedas, Nagano, Débora Satie, Cunha, Marcos Paulo Vieira, Neves, Patrícia Regina, Franco, Lucas Augusto Moyses, Moura, Maria Luísa Nascimento, Rizek, Camila Fonseca, Guimarães, Thais, Boszczowski, Ícaro, Rossi, Flávia, Levin, Anna S., Stabler, Richard A., Costa, Silvia F.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2019; Springer Nature B.V
• Subjects: Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; Case Report; Case reports; Colonization; Conjugation; E coli; Family Medicine; Gene sequencing; General Practice; Genomes; Identification methods; Infectious Diseases; Internal Medicine; Klebsiella; Medicine; Medicine & Public Health; Schistosomiasis; Brazil; Resistance; Antimicrobial susceptibility; Colistin; mcr-1
• ISSN: 0300-8126; 1439-0973
• DOI: 10.1007/s15010-019-01309-2

Case presentation We present a case report of a woman, concurrently colonized by polymyxin-resistant E. coli and K. pneumoniae . A Brazilian female patient, in her mid-fifties, was hospitalized with schistosomiasis. During hospitalization, polymyxin-resistant E. coli and K. pneumoniae were isolated from surveillance cultures. Methods Identification, antimicrobial susceptibility testings, PCR for mcr - 1 , plasmid transfer by conjugation and whole genome sequencing were performed. Results E. coli ST744 and K. pneumoniae ST101 carrying mcr -1 gene were described. Transconjugant E. coli was positive for mcr - 1 and IncX4 by PCR. The plasmid is a 33,304-base pair plasmid, and the mcr - 1 gene was the only antimicrobial resistance gene present in the plasmid. Conclusions This study presents a case report of a hospitalized woman, concurrently colonized by mcr - 1 -harboring E. coli ST744, a different ST from previously described in Brazil, and a K. pneumoniae ST101.