Effect of hydrogen-rich water on the Nrf2/ARE signaling pathway in rats with myocardial ischemia-reperfusion injury

• Published in: Journal of bioenergetics and biomembranes Vol. 51; no. 6; pp. 393 - 402
• Main Authors: Li, Liangtong, Liu, Tongtong, Liu, Li, Li, Shaochun, Zhang, Zhe, Zhang, Ruisha, Zhou, Yujuan, Liu, Fulin
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2019; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Biochemistry; Bioorganic Chemistry; Cardiomyocytes; Chemistry; Chemistry and Materials Science; Disease Models, Animal; Histology; Humans; Hydrogen; Hydrogen - metabolism; Immunohistochemistry; Injury prevention; Ischemia; Levels; Male; Morphology; mRNA; Myocardial ischemia; Myocardial Reperfusion Injury - genetics; NF-E2-Related Factor 2 - metabolism; Organic Chemistry; Oxidative stress; Proteins; Rats; Rats, Wistar; Reperfusion; Rodents; Signal Transduction; Signaling; Superoxide dismutase; Water - chemistry; Hydrogen-rich water; Oxidative stress; SOD; MDA; Nrf2/ARE signaling pathway; Myocardial ischemia-reperfusion injury
• ISSN: 0145-479X; 1573-6881
• DOI: 10.1007/s10863-019-09814-7

The effects of hydrogen-rich water on oxidative stress via the Nrf2/ARE signaling pathway were studied in rats with myocardial ischemia-reperfusion injury (MIRI). Sixty rats were randomly divided into a hydrogen-rich water group and a control group, with 30 rats in each group. The two groups were randomly divided into three groups: pre-ischemic period, ischemic period and reperfusion period. After the heart was removed, it was fixed in a Langendorff device and perfused with an oxygen-balanced 37 °C perfusate. The control group was perfused with Kreb’s-Ringers (K-R) solution, and the hydrogen-rich water group was perfused with K-R solution + hydrogen-rich water. The levels of mRNA and protein of Nrf2, NQO1, HO-1 and SOD-1 in cardiomyocytes were detected by RT-qPCR, immunohistochemistry (IHC) and Western blot analysis. SOD activity and MDA content were determined. Hydrogen-rich water increased the activation of the Nrf2/ARE signaling pathway, and the levels of mRNA and protein Nrf2, NQO1, HO-1 and SOD-1 were significantly increased ( P  < 0.05) in the ischemia-reperfusion period compared with the ischemic period. In the control group, the levels of mRNA and protein of Nrf2, NQO1, HO-1 and SOD-1 were significantly decreased ( P  < 0.05) in the ischemia-reperfusion period compared with the ischemic period. Compared with the ischemic period, the ischemia-reperfusion phase showed significantly increased SOD activity and significantly decreased MDA content in the hydrogen-rich water group, while SOD activity was significantly decreased, and MDA content was significantly increased in the control group ( P  < 0.05). Hydrogen-rich water can activate the Nrf2/ARE signaling pathway, alleviate ischemia-reperfusion injury in isolated rat hearts and reduce the oxidative stress level of myocardial tissue.