VvDAM-SVPs genes are regulated by FLOWERING LOCUS T (VvFT) and not by ABA/low temperature-induced VvCBFs transcription factors in grapevine buds
Main conclusion In deciduous fruit trees in which dormancy is induced by low temperatures, the expression of DORMACY-ASSOCIATED MADS-BOX genes (DAM) is regulated by CBF/DREB1 transcription factors. In Vitis vinifera , in which dormancy is induced by the photoperiod, VvDAM-SVPs gene expression is reg...
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Published in | Planta Vol. 253; no. 2; p. 31 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.02.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Main conclusion
In deciduous fruit trees in which dormancy is induced by low temperatures, the expression of
DORMACY-ASSOCIATED MADS-BOX
genes
(DAM)
is regulated by CBF/DREB1 transcription factors. In
Vitis vinifera
, in which dormancy is induced by the photoperiod,
VvDAM-SVPs
gene expression is regulated by
FLOWERING LOCUS T
(
VvFT
).
Using the sequences of the six peach (
Prunus persica
)
DORMACY-ASSOCIATED MADS-box
genes (
DAM
) as query, eight putative
DAM
genes belonging to the family of MADS-box transcription factors and related to the Arabidopsis floral regulators
SHORT VEGETATIVE PHASE
(
SVP
) and
AGAMOUS LIKE 24
(
AGL24
) were identified in the
V. vinifera
genome. Among these, five belong to the subfamily
SVP-like
genes which have been associated with the regulation of flowering and dormancy in annual and perennial plants, respectively. It has been proposed that they play a direct role in the induction and maintenance of endodormancy (ED) through the regulation of the
FLOWERING LOCUS T
(
FT
) gene. In the present study, it is demonstrated that in
V. vinifera
: (1)
VvDAM-SVPs
genes are not regulated by ABA/low temperature-induced VvCBFs transcription factors as described for other species of deciduous fruit trees. (2) A contrasting expression pattern between
VvDAM3-SVP
and
VvFT
was found under different experimental conditions related to the entry and exit of grapevine buds from ED. (3) Overexpression of
VvFT
in somatic grapevine embryos (SGE) repressed the expression of
VvDAM3-SVP
and
VvDAM4-SVP
. Taken together, the results suggest that
VvDAM3-SVP
could be associated with ED in grapevine buds, and that its expression could be regulated by
VvFT
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0032-0935 1432-2048 |
DOI: | 10.1007/s00425-020-03561-5 |