Genome analysis of Pollutimonas subterranea gen. nov., sp. nov. and Pollutimonas nitritireducens sp. nov., isolated from nitrate- and radionuclide-contaminated groundwater, and transfer of several Pusillimonas species into three new genera Allopusillimonas, Neopusillimonas, and Mesopusillimonas
Two facultatively anaerobic, chemoorganoheterotrophic bacterial strains, designated JR1/69-2-13 T and JR1/69-3-13 T , were isolated from nitrate- and radionuclide-contaminated groundwater (Ozyorsk town, South Urals, Russia). Both strains were found to be motile, Gram-stain negative rod-shaped neutro...
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Published in | Antonie van Leeuwenhoek Vol. 116; no. 2; pp. 109 - 127 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.02.2023
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Two facultatively anaerobic, chemoorganoheterotrophic bacterial strains, designated JR1/69-2-13
T
and JR1/69-3-13
T
, were isolated from nitrate- and radionuclide-contaminated groundwater (Ozyorsk town, South Urals, Russia). Both strains were found to be motile, Gram-stain negative rod-shaped neutrophilic, psychrotolerant bacteria that grow within the temperature range from 5–10 to 33 °C at 0–3 (0–5)% NaCl (w/v). The major cellular fatty acids were identified as C
16:0
, C
16:1
ω
7
c
, C
18:1
ω
7
c
and C
17:0
cyclo. The major polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol and unidentified aminophospholipids. The genomic G + C content of strains JR1/69-2-13
T
and JR1/69-3-13
T
was determined to be 57.2 and 57.9%, respectively. The 16S rRNA gene sequences of the strains showed high similarity between each other (98.6%) and to members of the genera
Pusillimonas
(96.8–98.4%) and
Candidimonas
(97.1–98.0%). The average nucleotide identity and digital DNA–DNA hybridization (dDDH) values among genomes of the new isolates and
Pusillimonas
and
Candidimonas
genomes were below 84.5 and 28.8%, respectively, i.e., below the thresholds for species delineation. Based on the phylogenomic, phenotypic and chemotaxonomic characterisation, we propose assignment of strains JR1/69-3-13
T
(= VKM B-3223
T
= KCTC 62615
T
) and JR1/69-2-13
T
(= VKM B-3222
T
= KCTC 62614
T
) to a new genus
Pollutimonas
as the type strains of two new species,
Pollutimonas subterranea
gen. nov., sp. nov. and
Pollutimonas nitritireducens
sp. nov., respectively. As a result of the taxonomic revision of the genus
Pusillimonas
, three novel genera,
Allopusillimonas
,
Neopusillimonas
, and
Mesopusillimonas
are also proposed; and
Candidimonas bauzanensis
is reclassified as
Pollutimonas bauzanensis
comb. nov. Genome analysis of the new isolates suggested molecular mechanisms of their adaptation to an environment highly polluted with nitrate and radionuclides. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-6072 1572-9699 |
DOI: | 10.1007/s10482-022-01781-1 |