Effects of vitamin B12 on methotrexate hepatotoxicity: evaluation of receptor-interacting protein (RIP) kinase

In the study, we aimed to show the effects of vitamin B12 on the necrosis caused by methotrexate (MTX), a folic acid antagonist. Thirty-two rats were randomly assigned to four groups of eight rats per group. Control ( n = 8), Vit B12 ( n = 8) 3 μg/kg/ip B12 (15 days) per day throughout the experimen...

Full description

Saved in:
Bibliographic Details
Published inNaunyn-Schmiedeberg's archives of pharmacology Vol. 393; no. 12; pp. 2473 - 2480
Main Authors Karabulut, Derya, Ozturk, Emel, Kuloglu, Nurhan, Akin, Ali Tuğrul, Kaymak, Emin, Yakan, Birkan
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2020
Springer Nature B.V
Subjects
Antioxidants
Biomedical and Life Sciences
Biomedicine
Cyclooxygenase-2
Experiments
Folic acid
Hepatotoxicity
Immunohistochemistry
Kinases
Methotrexate
Neurosciences
Original Article
Oxidants
Pharmacology/Toxicology
Protein kinase
Tumor necrosis factor-α
Vitamin B12
Receptor-interacting protein kinase
Methotrexate
Vitamin B12
Online AccessGet full text

Cover

More Information
Summary:In the study, we aimed to show the effects of vitamin B12 on the necrosis caused by methotrexate (MTX), a folic acid antagonist. Thirty-two rats were randomly assigned to four groups of eight rats per group. Control ( n = 8), Vit B12 ( n = 8) 3 μg/kg/ip B12 (15 days) per day throughout the experiment, MTX ( n = 8) injected with a single dose of 20 mg/kg/ip MTX on 8th day of experiment, MTX + Vit B12 ( n = 8) injected with a single dose of 20 mg/kg ip methotrexate on 8th day of experiment + 3 μg/kg/ip Vit B12 (15 days) per day throughout the experiment. Oxidant (TOS)/antioxidant (TAS) system, TNF-α and TGF-β levels, AST and ALT, serum vitamin B12 levels were determined in the tissue. Cyclooxygenase-2 (Cox-2), receptor-interacting protein kinase 1 (RIP1) and 3 (RIP3) immunohistochemistry were applied to the liver tissue. TOS increased; TAS decreased; TNF-α and TGF-β levels increased; AST and ALT levels changed after MTX hepatotoxicity. Vit B12 decreased significantly. COX-2, RIP1, and RIP3 immunoreactivity increased. Vit B12 showed improvement in all of the negative results. Vit B12 is an important supplement to be used against necrosis in tissue after MTX hepatotoxicity.
ISSN:0028-1298
1432-1912
DOI:10.1007/s00210-020-01992-1