Vaccaria n-Butanol Extract Lower the Production of Proinflammatory Cytokines and the Infection Risk of T. spiralis In Vivo

• Published in: Acta parasitologica Vol. 64; no. 3; pp. 520 - 527
• Main Authors: Xu, Fei, Hou, Bao, Zhu, Xuexue, Liu, Yixiao, Shi, Xuelin, Li, Shuangshuang, Li, Zhongjie, Cai, Weiwei, Zhou, Yuetao, Qiu, Liying
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2019; Springer Nature B.V
• Subjects: Animal Systematics/Taxonomy/Biogeography; Animals; Anthelmintic agents; Anti-inflammatory agents; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - analysis; Antiparasitic agents; Biological activity; Biomedical and Life Sciences; Biomedicine; Butanol; Cell activation; Chemical compounds; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - immunology; Cyclooxygenase-2; Cytokines; Ecology; Evaluation; Health risks; Humans; IL-1β; Immunohistochemistry; Infections; Inflammation; Interleukin 6; Interleukin-6 - genetics; Interleukin-6 - immunology; Larvae; Male; Medical Microbiology; Medical treatment; Mice; Mice, Inbred ICR; Microbiology; Muscles; Original Paper; Parasitology; Pharmacology; Plant Extracts - administration & dosage; Plant Extracts - analysis; Seeds; Semen; Trichinella - drug effects; Trichinella - physiology; Trichinellosis; Trichinellosis - drug therapy; Trichinellosis - genetics; Trichinellosis - immunology; Trichinellosis - parasitology; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-α; Vaccaria; Vaccaria - chemistry; Zoonoses; Trichinellosis; Vaccarin; Zoonosis; Proinflammatory cytokines; Vaccariae semen; Hypaphorine
• ISSN: 1230-2821; 1896-1851
• DOI: 10.2478/s11686-019-00064-6

Introduction Trichinellosis is a severe zoonosis involving the activation of inflammatory cells, accompanied by the prominent expressions of proinflammatory cytokines in the host. Semen vaccariae, the seeds of Vaccaria segetalis (Neck.) Garcke. ex Asch. (Caryophyllaceae), is a famous traditional herb that is rich in vaccaria n-butanol extract (VNE). Vaccarin is one major active component of VNE, and it is reported in the treatment of stranguria disease. Hypaphorine is another main active component of VNE and has good anti-inflammatory effect, whereas the potential bioactivity of VNE in trichinellosis treatment is still unknown. Materials and methods This study was designed to evaluate the potential anthelmintic and anti-inflammatory activity of VNE toward T. spiralis infection. ICR mice were used to assess the effect of VNE on repression larvae and adult worms in vivo. Immunohistochemistry analysis was performed to evaluate the expression levels of IL-1β, IL-6, TNF-α, and COX-2. Results Our results showed that VNE could effectively depress the expressions of proinflammatory cytokines, including IL-1β, IL-6, TNF-α, and COX-2. The adult worms were decreased by 79.53%, while the muscle larvae were diminished by 77.70% as compared to the control. Conclusion These results demonstrated that VNE may be a promising therapeutic agent against the inflammation and diseases caused by T. spiralis infection.