The prevalence of plasmid-mediated quinolone resistance genes among Escherichia coli strains isolated from urinary tract infections in southwest Iran
Background The extensive and inappropriate use of quinolones, which are frequently used as an effective treatment for urinary tract infection (UTI) patients, has led to resistance to these antibiotics. This study was designed to determine the prevalence of quinolones resistance and the presence of p...
Saved in:
Published in | Molecular biology reports Vol. 49; no. 5; pp. 3757 - 3763 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.05.2022
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Background
The extensive and inappropriate use of quinolones, which are frequently used as an effective treatment for urinary tract infection (UTI) patients, has led to resistance to these antibiotics. This study was designed to determine the prevalence of quinolones resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among extended-spectrum beta-lactamase (ESBL)-producing
Escherichia coli
isolates.
Methods
One hundred and fourteen
E. coli
isolates were collected from patients’ urine samples. The susceptibility of isolates to selected antibiotics was tested by the Kirby-Bauer disk diffusion method. ESBL-producing isolates were identified phenotypically using a combination disk test. Using specific primers, the frequency of
aac (6’)-Ib
,
qnrA
,
qnrB
,
qnrC
,
qnrD
,
qnrS
, and
qepA
genes was investigated by polymerase chain reaction (PCR).
Results
Among 26 ESBL-producing isolates, the highest resistance rate was observed toward nalidixic acid (80.8%) and ciprofloxacin (61.5%), respectively. Ninety-seven (85%) of all isolates harbored at least one PMQR gene, the most frequent one being
aac(6’)-Ib-cr
variant (47.4%). Coexistence of
aac(6’)-Ib-cr
variant and
qnrB
were the most broadly distributed genotype among quinolone resistance isolates. Notably, none of the isolates contained the
qnrC
,
qnrD
, and
qepA
genes.
Conclusions
Our results highlight the significant prevalence of PMQR genes in ESBL-producing
E. coli
isolates in this region. Also, the
aac (6’)-Ib-cr
variant was the most frequent gene, particularly in ESBL positive isolates. A regular periodic monitoring program is needed to control and hinder the more spread of antibiotic resistance phenomenon and contributed genes among UTI-causing
E. coli
isolates. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-022-07215-5 |