Cryopreservation of filamentous micromycetes and yeasts using perlite

The viability, growth and morphology of 48 strains of Ascomycota (including 17 yeasts) and 20 strains of Zygomycota were determined after a 2-d and then after 1-year storage in liquid nitrogen using a new cryopreservation method with perlite as a particulate solid carrier. In case of Ascomycota, 45...

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Published inFolia microbiologica Vol. 52; no. 2; pp. 153 - 157
Main Authors Homolka, L, Lisá, L, Kubatová, A, Valqová, M, Janderová, B, Nerud, F
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.01.2007
Subjects
Aluminum Oxide
Ascomycota
Ascomycota - growth & development
Cryopreservation
Cryopreservation - methods
Fungi
Fungi - growth & development
Liquid nitrogen
Low temperature physics
Microbiology
Micromycetes
Morphology
Nitrogen
Perlite
Silicon Dioxide
Survival
Yeast
Yeasts
Zygomycota
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Summary:The viability, growth and morphology of 48 strains of Ascomycota (including 17 yeasts) and 20 strains of Zygomycota were determined after a 2-d and then after 1-year storage in liquid nitrogen using a new cryopreservation method with perlite as a particulate solid carrier. In case of Ascomycota, 45 strains (94 %) out of 48 survived both 2-d and 1-year storage in liquid nitrogen, respectively. In case of Zygomycota, all 20 strains survived both storage. In addition, 3 strains of Basidiomycota counted among yeasts were tested and all survived the 1 year storage. In all surviving cultures no negative effects of cryopreservation by this method have been observed after 1-year of storage in liquid nitrogen. The results indicate that the perlite protocol can be successfully used for cryopreservation of taxonomically different groups of fungi and also for fungi which failed to survive other routinely used preservation procedures.
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ISSN:0015-5632
1874-9356
DOI:10.1007/BF02932154