Molecular phylogeny, pathogenic variability and phytohormone production of Fusarium species associated with bakanae disease of rice in temperate agro-ecosystems

• Published in: Molecular biology reports Vol. 48; no. 4; pp. 3173 - 3184
• Main Authors: Mohiddin, F. A., Majid, Rukhsanah, Bhat, Arif Hussain, Dar, M. S., Shikari, Asif B., Sofi, Najeebur Rehman, Nabi, Sajad un, Hamid, Aflaq, Ahanger, M. Ashraf, Bhat, F. A., Hussain, Ashaq, Bhat, N. A.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2021; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Blight; Cultivars; Fusarium; Gibberellins; Histology; Indoleacetic acid; Life Sciences; Morphology; Original Article; Pathogenicity; Phylogeny; Phytohormones; Rice; Seeds; Species; Virulence; Phytohormones; spp; Phylogeny; TEF-1α; Bakanae; Rice; Fusarium spp
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06337-6

Bakanae is the emerging disease threating the rice cultivation globally. Yield reduction of 4–70% is recorded in different parts of the world. A total of 119 Fusarium isolates were collected from rice plants at different geographical locations and seeds of different rice cultivars. The isolates were evaluated for morphological, biochemical and pathogenic diversity. The amplification of TEF-1α gene was carried out for exploring the species spectrum associated with the cultivated and pre-released rice varieties. The production of gibberellin varied from 0.53 to 2.26 µg/25 ml, while as that of Indole acetic acid varied from 0.60 to 3.15 µg/25 ml among the Fusarium isolates. The phylogenetic analysis identified 5 different species of the genus Fusarium viz. Fusarium fujikuroi , F. proliferatum , F. equiseti , F.oxysporum and F. persicinum after nucleotide blasting in NCBI. Only two Fusarium spp. F. fujikuroi and F. proliferatum were found to be pathogenic under virulence assays of the isolates. The isolates showed a considerable variation in morphological and pathogenic characters. The isolates were divided into different groups based on morphology and pathogenicity tests. The isolates showed a considerable variation in morphology, phytohormone profile and virulence indicative of population diversity. Three species F. equiseti , F.oxysporum and F. persicinum which have not been reported as pathogens of rice in India were found to be associated with bakanae disease of rice, however their pathogenicity could not be established.