Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems

• Published in: Semina. Ciências agrárias : revista cultural e científica da Universidade Estadual de Londrina Vol. 37; no. 5; pp. 3125 - 3132
• Main Authors: Bicalho, Juliana Marques, Fonseca Júnior, Antônio Augusto, Oliveira, Cairo Henrique Sousa de, Resende, Cláudia Fideles, Kassar, Telissa Da Cunha, Galinari, Grazielle Cossenzo Florentino, Oliveira, André Penido, Orzil, Livia De Lima, Reis, Jenner Karlisson Pimenta dos, Leite, Rômulo Cerqueira
• Format: Journal Article
• Language: English
• Published: Universidade Estadual de Londrina 26.10.2016
• Subjects: BLV; Bovinos; BTV; Reprodução; Útero
• ISSN: 1676-546X; 1679-0359
• DOI: 10.5433/1679-0359.2016v37n5p3125

Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance.