Endogenous Regulatory T Cells Promote M2 Macrophage Phenotype in Diabetic Stroke as Visualized by Optical Imaging

• Published in: Translational stroke research Vol. 12; no. 1; pp. 136 - 146
• Main Authors: Cai, Yu, Xu, Ting-Ting, Lu, Chun-Qiang, Ma, Yuan-Yuan, Chang, Di, Zhang, Yi, Gu, Xiao-Chun, Ju, Shenghong
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2021; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Brain; Cardiology; Cloning; Cytokines; Diabetes; Flow cytometry; Hyperglycemia; Inflammation; Ischemia; Lymphocytes; Magnetic resonance imaging; Neurology; Neurosciences; Neurosurgery; Original Article; Polyclonal antibodies; Proteins; Vascular Surgery; United States > US; China; Germany; Optical imaging; Type 2 diabetes mellitus; Ischemic stroke; Regulatory T cells; M2 macrophages/microglia
• ISSN: 1868-4483; 1868-601X
• DOI: 10.1007/s12975-020-00808-x

Regulatory T cells (Tregs) play an immunosuppressive role in various diseases, yet their function remains controversial in stroke and obscure in diabetic stroke. In the present study, Tregs were found downregulated in the peripheral blood of type 2 diabetes mellitus (T2DM) stroke models and patients compared with controls. In ischemic stroke mice (both T2DM and wild type), endogenous Tregs boosted by CD28SA increased CD206+ M2 macrophage/microglia cells, decreased infarct volumes, and improved neurological recovery. Our results demonstrated the potential of boosting Tregs for treating T2DM stroke. Furthermore, we utilized an optical imaging probe (IRD-αCD206) to target M2 macrophage/microglia cells and demonstrated its effect in visualizing M2 macrophage/microglia cells in vivo in ischemic brain tissue.