Description of Gemmobacter aestuarii sp. nov., isolated from estuarine surface water and reclassification of Cereibacter changlensis as Gemmobacter changlensis Chen et al. 2013

• Published in: Archives of microbiology Vol. 202; no. 5; pp. 1035 - 1042
• Main Authors: Hameed, Asif, Shahina, Mariyam, Lin, Shih-Yao, Chen, Wen-Ming, Hsu, Yi-Han, Lai, Wei-An, Young, Chiu-Chung
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2020; Springer Nature B.V
• Subjects: Bacteriochlorophyll; Biochemistry; Biomedical and Life Sciences; Biotechnology; Carbon dioxide; Cell Biology; Coenzyme Q10; Deoxyribonucleic acid; DNA; Ecology; Estuaries; Fatty acids; Genomes; Hydrogen sulfide; Lecithin; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; Nucleotide sequence; Nucleotides; Original Paper; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Photosynthesis; Quinones; Reaction centers; Reclassification; rRNA 16S; Surface water; Tyrosine; Ubiquinone; Bacteriochlorophyll; RuBisCO; Carbon monoxide dehydrogenase; Light-harvesting complex; Rhodobacteraceae
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-020-01809-y

A Gram-stain-negative, tyrosine-metabolizing, non-motile, strictly aerobic, non-spore-forming, rod-shaped marine bacterium, designated strain CC-PW-75 T , was isolated from the estuarine water off Pintung, Taiwan. Strain CC-PW-75 T formed a distinct phyletic lineage associated with Gemmobacter species, sharing the highest 16S rRNA gene sequence similarity with G. megaterium CF17 T and G. straminiformis CAM-8 T (96.0% each) followed by G. aquatilis IFAM 1031 T and G. nectariphilus AST4 T (95.8% each). Analysis of the draft genome (3.76 Mbp) revealed the presence of genes encoding light-harvesting complexes, photosynthetic reaction centers and proteins involved in the metabolism of CO, CO 2 , HCO 3 ‒ and H 2 S. However, bacteriochlorophyll a was not detected. Average nucleotide identity values between the genome sequence of CC-PW-75 T and the related Gemmobacter species ( n  = 6) were estimated to be 72.8–76.3%. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid in major amounts, and phosphatidylmonomethylethanolamine, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid in minor amounts. C 18:1 ω 7 c and/or C 18:1 ω 6 c, C 18:0 and C 18:1 ω 7 c 11-methyl were identified to be major fatty acids. The DNA G + C content was 66.2 mol% (draft genome sequence). Ubiquinone-10 (Q-10) was the sole respiratory quinone. Based on the polyphasic taxonomic evidence, CC-PW-75 T is most likely a novel species of the genus Gemmobacter , affiliated to the family Rhodobacteraceae , for which the name Gemmobacter aestuarii sp. nov. is proposed. The type strain is CC-PW-75 T (= JCM 19754 T  = BCRC 80759 T ). Also, we propose the reclassification of Cereibacter changlensis as Gemmobacter changlensis Chen et al. 2013 using the polyphasic data presented in this study.