Minocycline as heart conditioning agent in experimental type 2 diabetes mellitus — an antibacterial drug in heart protection

• Published in: Naunyn-Schmiedeberg's archives of pharmacology Vol. 395; no. 4; pp. 429 - 444
• Main Authors: Sobot, Nikola M., Sobot, Tanja S., Jeremic, Jovana N., Bolevich, Sergey B., Bolevich, Stefani S., Mitrovic, Slobodanka Lj, Fisenko, Vladimir P., Inic, Sofija G., Samanovic, Andjela D. Milojevic, Rankovic, Marina R., Srejovic, Ivan M., Zivkovic, Vladimir I., Jakovljevic, Vladimir Lj
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2022; Springer Nature B.V
• Subjects: Antibiotics; Antioxidants; Apoptosis; Biomedical and Life Sciences; Biomedicine; Cardiac conditioning; Cardiovascular diseases; Coronary artery disease; Diabetes; Diabetes mellitus (non-insulin dependent); Heart attacks; Heart diseases; Inflammation; Ischemia; Minocycline; Myocardial infarction; Neurosciences; Original Article; Oxidative stress; Pharmacology/Toxicology; Recovery of function; Reperfusion; Superoxide anions; Ventricle; Type 2 diabetes; Minocycline; Oxidative stress; Heart conditioning; Cardiodynamics
• ISSN: 0028-1298; 1432-1912
• DOI: 10.1007/s00210-021-02179-y

Cardiovascular diseases, and among them certainly myocardial infarction, remain leading cause of death worldwide. Diabetes increases risk of occurrence as well as adverse outcome of myocardial infarction. Conditioning maneuvers are the most attractive method for alleviating both the consequences of ischemia and reperfusion. Minocycline is a tetracycline derivative which exerts antioxidant, anti-inflammatory, and anti-apoptotic effects. The aim of this study was to assess the protective ability of preconditioning and postconditioning of isolated hearts from healthy and rats with experimentally induced type 2 diabetes with minocycline on functional recovery and redox status after ischemia and reperfusion. The hearts from healthy and diabetic rats were excised and retrogradely perfused according to the Langendorff technique. Using sensor in the left ventricle, the cardiodynamic parameters were recorded and in the samples of the coronary venous effluent oxidative stress biomarkers were analyzed. Minocycline was injected directly into the coronary vessels, in preconditioning 5 min before global ischemia, and in postconditioning during the first 5 min of reperfusion. Results of this study clearly show beneficial effects of minocycline applied both before ischemia and in the first minutes of reperfusion fashion in both healthy and diabetic rat hearts. The most prominent protective effect regarding oxidative stress is related to the decreased production of superoxide anion radical due postconditioning with minocycline in diabetic hearts. Cardiodynamic parameters were significantly improved in minocycline conditioned groups. Superoxide anion radical stands out as the most susceptible to changes induced by minocycline.