Corallincola spongiicola sp. nov., isolated from sponge

• Published in: Antonie van Leeuwenhoek Vol. 113; no. 5; pp. 643 - 650
• Main Authors: Choi, Heejae, Im, Wan-Taek, Park, Jin-Sook
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.05.2020; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; Fatty acids; Genomes; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nucleotide sequence; Original Paper; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Plant Sciences; Quinones; rRNA 16S; Soil Science & Conservation; Polyphasic taxonomy; Corallincola spongiicola; Genome sequence; 16S rRNA gene
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-019-01371-8

A gram-negative, motile, strictly aerobic, and rod-shaped bacterium designated 176GS2-150 T was isolated from the sponge Hymeniacidon sinapium . The taxonomic position of the novel isolate was confirmed using the polyphasic approach. Strain 176GS2-150 T grew well at 25 °C on marine agar. Based on its 16S rRNA gene sequence, we showed that strain 176GS2-150 T belongs to the family Psychromonadaceae and class Gammaproteobacteria and is related to Corallincola platygyrae JLT2006 T (96.84% sequence similarity). The G + C content of the genomic DNA was 49.0 mol%. The assembled draft genome of strain 176GS2-150 T was 4.2 Mbp and consisted of 14 contigs. The major respiratory quinone was Q-8, and the major fatty acids were summed feature 3 (comprising C 16 :1 ω 6 c and/or C 16:1 ω 7 c ), summed feature 8 (comprising C 18 :1 ω 7 c and/or C 18:1 ω 6 c ), C 17:0 iso, C 16:0 , and C 15:0 iso. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, 3 unidentified phospholipids, and 1 unidentified polar lipid. On the basis of the genotypic and phenotypic characteristics, strain 176GS2-150 T can be placed as a new species within the genus Corallincola ; the name Corallincola spongiicola sp. nov. has been proposed, with type strain 176GS2-150 T (= KACC 19890 T  = LMG 31317 T ).