Molecular characterization of fungal endophyte diversity isolated from Aconitum heterophyllum: a critically endangered medicinal plant of Kashmir Himalaya

• Published in: International microbiology Vol. 26; no. 3; pp. 651 - 662
• Main Authors: Hafeez, Sabira, Yaqoob, Sabba, Magray, Aqib Rehman, Kamili, Azra N., Ganai, Bashir Ahmad
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2023; Spanish Society for Microbiology
• Subjects: Aconitum; Applied Microbiology; Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; Endangered plants; Endangered species; Endophytes; Eukaryotic Microbiology; Fungi; Herbal medicine; Life Sciences; Medical Microbiology; Medicinal plants; Microbial Ecology; Microbiology; Nucleotide sequence; Phylogeny; Plant tissues; Plants (botany); Endophytes; Microbiology; Kashmir; Molecular identification; Medicinal plant
• ISSN: 1618-1905; 1139-6709; 1618-1905
• DOI: 10.1007/s10123-023-00331-7

Aconitum heterophyllum is a rare perennial herb from Kashmir Himalayas. Due to its threatened status and dependence on its environment, the plant was examined for any potential endophytes, which is of utmost importance for bioprospection. In the current study, endophytic fungal diversity associated with A. heterophyllum was examined, and 328 fungal isolates were found in the plant’s leaf, stem, and root tissues. Twelve (12) endophytic fungal species were identified utilizing, molecular analysis of the nuclear ribosomal DNA Internal Transcribes Spacer (ITS), rLSU, and rSSU sequences. Maximum likelihood analysis was used to determine the phylogenetic connection between each isolate. The genera Arthrinium , Chaetomium , Purpureocillium , Alternaria , Penicillium , Aspergillus , Cladosporium , and Bjerkandera species dominated the ascomycete and basidiomycete fungal endophytes.