Isolation and characterization of detergent-compatible amylase-, protease-, lipase-, and cellulase-producing bacteria

• Published in: Brazilian journal of microbiology Vol. 54; no. 2; pp. 725 - 737
• Main Authors: Inan Bektas, Kadriye, Nalcaoğlu, Aleyna, Ceylan, Esma, Colak, Disat Nigar, Caglar, Pınar, Agirman, Sevda, Sivri, Nur Sena, Gunes, Sueda, Kaya, Alanur, Canakci, Sabriye, Belduz, Ali Osman
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2023; Springer Nature B.V
• Subjects: Amylases; Bacteria; Bacterial Proteins - chemistry; Biomedical and Life Sciences; Biotechnology and Industrial Microbiology - Research Paper; Cellulase; Cellulases; Cellulose; Compatibility; Detergent industry; Detergents; Detergents - chemistry; Enzymes; Food Microbiology; Household wastes; Industrial areas; Life Sciences; Lipase; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microorganisms; Morphology; Mycology; Peptide Hydrolases; Protease; Proteinase; RNA, Ribosomal, 16S - genetics; rRNA 16S; Screening; Soil contamination; Soil pollution; Detergent compatible; Amylase; Lipase; Cellulase; Protease
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-023-00944-0

Detergent-compatible enzymes are the new trend followed by most in the detergent industry. Cellulases, lipases, proteases, and amylases are among the enzymes frequently used in detergents. Detergent-compatible enzymes can be obtained from many organisms, but the stability, cheapness, and availability of microbial enzymes make them preferable in industrial areas. In the present study, soil samples contaminated with household waste were collected from different regions of Trabzon (Turkey) for amylase-, cellulase-, protease-, and lipase-producing bacteria. A total of 55 bacterial isolates differing in colony morphology were purified from the samples and 25 of the isolates gave positive results in enzyme screening. The enzyme screening experiments revealed that 10 isolates produced amylase, 9 produced lipase, 7 produced cellulase, and 6 produced protease. While 2 isolates showed both protease and lipase activity, for 2 different isolates cellulose and amylase activity were detected together. It was also observed that one isolate, C37PLCA, produced all four enzymes. The morphological, physiological, and biochemical analyses of the bacteria from which we obtained the enzymes were performed and species close to them were determined using 16S rRNA sequences. Based on the results obtained, our enzymes show tremendous promise for the detergent industry.