Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: A study of 129 samples

• Published in: Clinical biochemistry Vol. 41; no. 14-15; pp. 1204 - 1210
• Main Authors: Moiz, Bushra, Rasool Hashmi, Mashhooda, Sadaf, Shahmina
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2008
• Subjects: Adolescent; Adult; Aged; Blood Glucose; Chromatography; Chromatography, Affinity - methods; Chromatography, Affinity - standards; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange - methods; Chromatography, Ion Exchange - standards; Diabetes; Diabetes Mellitus - blood; Fasting - blood; Female; Glycated Hemoglobin A - analysis; HbA1c; HbD; Hemoglobin variant; Hemoglobins, Abnormal - analysis; Humans; Male; Middle Aged; Diabetes; HbD; Hemoglobin variant; HbA1c; Chromatography
• ISSN: 0009-9120; 1873-2933; 1873-2933
• DOI: 10.1016/j.clinbiochem.2008.06.015

To compare ion exchange and boronate affinity chromatography for HbA1c estimation in patients with type I and II diabetes having hemoglobin D. Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA1c estimation in patients with homozygous and heterozygous D disease. Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA1c estimation. The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.