Molecular Characterization and Phylogeny of Taenia hydatigena and Echinococcus granulosus from Iranian Sheep and Cattle Based on COX1 Gene
Hydatid cyst , the larval stage of Echinococcus granulosus , and Cysticercus tenuicollis , the larval stage of Taenia hydatigena , are prevalent in domestic, livestock, and wild ruminants. The main goal of this research was to identify the isolates of E. granulosus and C. tenuicollis by partial sequ...
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Published in | Current microbiology Vol. 78; no. 4; pp. 1202 - 1207 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.04.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Hydatid cyst
, the larval stage of
Echinococcus granulosus
, and
Cysticercus tenuicollis
, the larval stage of
Taenia hydatigena
, are prevalent in domestic, livestock, and wild ruminants. The main goal of this research was to identify the isolates of
E. granulosus
and
C. tenuicollis
by partial sequencing with PCR amplification of the cytochrome C oxidase 1 (
COX1
) gene. During a routine veterinary inspection at a Chabahar city slaughterhouse, two samples of
hydatid cysts
from sheep's liver and cattle's lung and two samples of
C. tenuicollis
from sheep's liver were collected. After DNA extraction, the fragment of the
COX1
gene was amplified by the PCR method. Sample sequences were modified and synchronized by Chromas and CLC genomic workbench 11 software. Sequence analysis was carried out by BLAST algorithms and GenBank databases. Phylogenetic trees were performed using MEGA 7 software and the neighbor-joining and maximum likelihood method for
T. hydatigena
and
E. granulousus
. The result indicated that the main genotype of parasites and the amplified fragment size were G1 and approximately 455 bp, respectively. The analysis of phylogenetic trees based on nucleic acid for four samples showed that there was a common ancestor. However, the shift in nucleotides in the two isolates in
E. granulosus
and the two isolates of
T. hydatigena
were non-synonymous type and synonymous type, respectively. The present study showed that the dominant genotype in all isolates was G1 and this report was similar to other studies in Iran and the world. Also, the partial
COX1
gene sequence was matched with
T. hydatigena
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0343-8651 1432-0991 |
DOI: | 10.1007/s00284-021-02377-0 |