Intramural Distributions of GAGs and Collagen vs. Opening Angle of the Intact Porcine Aortic Wall

• Published in: Annals of biomedical engineering Vol. 50; no. 2; pp. 157 - 168
• Main Authors: Ghadie, Noor M., St-Pierre, Jean-Philippe, Labrosse, Michel R.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.02.2022; Springer Nature B.V
• Subjects: Animals; Aorta; Aorta, Thoracic - metabolism; Aortic arch; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Biophysics; Circumferences; Classical Mechanics; Collagen; Collagen - metabolism; Correlation; Elastin; Elastin - metabolism; Glycosaminoglycans; Glycosaminoglycans - metabolism; Heterogeneity; Original Article; Residual stress; Swine; Thorax; Thorax - metabolism; Opening angle; Aorta; Glycosaminoglycans; Collagen; Residual stress
• ISSN: 0090-6964; 1573-9686
• DOI: 10.1007/s10439-022-02901-8

The heterogeneity and contribution of collagen and elastin to residual stresses have been thoroughly studied, but more recently, glycosaminoglycans (GAGs) also emerged as potential regulators. In this study, the opening angle of aortic rings (an indicator of circumferential residual stresses) and the mural distributions of sulfated GAGs (sGAG), collagen, and elastin were quantified in the ascending, aortic arch and descending thoracic regions of 5- to 6-month-old pigs. The opening angle correlated positively with the aortic ring’s mean radius and thickness, with good and moderate correlations respectively. The correlations between the sGAG, collagen, elastin, and collagen:sGAG ratio and the opening angle were evaluated to identify aortic compositional factors that could play roles in regulating circumferential residual stresses. The total collagen:sGAG ratio displayed the strongest correlation with the opening angle ( r = − 0.715, p < 0.001), followed by the total sGAG content which demonstrated a good correlation ( r = 0.623, p < 0.001). Additionally, the intramural gradients of collagen, sGAG and collagen:sGAG correlated moderately with the opening angle. We propose that, in addition to the individual role sGAG play through their content and intramural gradient, the interaction between collagen and sGAG should be considered when evaluating circumferential residual stresses in the aorta.