Neurohumoral interactions contributing to renal vasoconstriction and decreased renal blood flow in heart failure

• Published in: American journal of physiology. Regulatory, integrative and comparative physiology Vol. 317; no. 3; pp. R386 - R396
• Main Authors: Ramchandra, Rohit, Xing, Daniel T, Matear, Marcus, Lambert, Gavin, Allen, Andrew M, May, Clive N
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.09.2019
• Subjects: Adrenergic alpha-1 Receptor Agonists - pharmacology; Adrenergic receptors; Angiotensin; Angiotensin II; Angiotensin II Type 1 Receptor Blockers - pharmacology; Animals; Blood flow; Blood Pressure - physiology; Cardiac Pacing, Artificial; Conductance; Congestive heart failure; Electrical stimuli; Female; Heart; Heart - innervation; Heart failure; Heart Failure - complications; Heart Failure - etiology; Heart Rate - physiology; Hemodynamics; Irbesartan - pharmacology; Kidney - blood supply; Kidney - innervation; Kidney - metabolism; Kidneys; Nerves; Norepinephrine; Norepinephrine - metabolism; Norepinephrine - pharmacology; Phenylephrine; Phenylephrine - pharmacology; Receptor, Angiotensin, Type 1 - physiology; Receptors; Receptors (physiology); Renal failure; Renal plexus; Renin; Renin - blood; Renin - metabolism; Sheep; Stimulation; Sympathetic nerves; Vasoconstriction; Vasoconstrictor Agents - pharmacology; cardiorenal syndrome type 2; heart failure; renal spillover; renal sympathetic nerve activity
• ISSN: 0363-6119; 1522-1490
• DOI: 10.1152/ajpregu.00026.2019

In heart failure (HF), increases in renal sympathetic nerve activity (RSNA), renal norepinephrine spillover, and renin release cause renal vasoconstriction, which may contribute to the cardiorenal syndrome. To increase our understanding of the mechanisms causing renal vasoconstriction in HF, we investigated the interactions between the increased activity of the renal nerves and the renal release of norepinephrine and renin in an ovine pacing-induced model of HF compared with healthy sheep. In addition, we determined the level of renal angiotensin type-1 receptors and the renal vascular responsiveness to stimulation of the renal nerves and α -adrenoceptors. In conscious sheep with mild HF (ejection fraction 35%-40%), renal blood flow (276 ± 13 to 185 ± 18 mL/min) and renal vascular conductance (3.8 ± 0.2 to 3.1 ± 0.2 mL·min ·mmHg ) were decreased compared with healthy sheep. There were increases in the burst frequency of RSNA (27%), renal norepinephrine spillover (377%), and plasma renin activity (141%), whereas the density of renal medullary angiotensin type-1 receptors decreased. In anesthetized sheep with HF, the renal vasoconstrictor responses to electrical stimulation of the renal nerves or to phenylephrine were attenuated. Irbesartan improved the responses to nerve stimulation, but not to phenylephrine, in HF and reduced the responses in normal sheep. In summary, in HF, the increases in renal norepinephrine spillover and plasma renin activity are augmented compared with the increase in RSNA. The vasoconstrictor effect of the increased renal norepinephrine and angiotensin II is offset by reduced levels of renal angiotensin type-1 receptors and reduced renal vasoconstrictor responsiveness to α -adrenoceptor stimulation.