TGF-β2-induced circ-PRDM5 regulates migration, invasion, and EMT through the miR-92b-3p/COL1A2 pathway in human lens epithelial cells

• Published in: Journal of molecular histology Vol. 53; no. 2; pp. 309 - 320
• Main Authors: Huang, Pengcheng, Hu, Yao, Duan, Yuping
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2022; Springer Nature B.V
• Subjects: Age; Biomedical and Life Sciences; Biomedicine; Cataracts; Cell Biology; Cell culture; Cell migration; Cell Movement - genetics; Cell Proliferation; Cell viability; Cells, Cultured; Collagen (type I); Collagen Type I - genetics; Collagen Type I - metabolism; Developmental Biology; Epithelial cells; Epithelial Cells - metabolism; Epithelial-Mesenchymal Transition - genetics; Eye surgery; Gene expression; Histology; Humans; Lens, Crystalline - metabolism; Life Sciences; Mesenchyme; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; mRNA; Original Paper; Proteins; RNA, Circular - biosynthesis; RNA, Circular - physiology; Transforming Growth Factor beta2 - metabolism; Transforming Growth Factor beta2 - pharmacology; Western blotting; Wound healing; PCO; TGF-β2; miR-92b-3p; circ-PRDM5; COL1A2
• ISSN: 1567-2379; 1567-2387
• DOI: 10.1007/s10735-021-10053-7

CircRNA circ-PRDM5 ( PR/SET domain 5 ) (circ-PRDM5) is overexpressed in age-related cataracts. Nevertheless, the biological role of circ-PRDM5 in posterior capsule opacities (PCO) (a common complication after cataract surgery) is unclear. Human lens epithelial cells SRA01/04 (LECs) were stimulated with TGF-β2 (transforming growth factor beta-2) to mimic the PCO model in vitro. Cell viability, migration, and invasion were determined by MTT, transwell, or wound-healing assays. Protein levels of EMT (epithelial-to-mesenchymal transition) markers and COL1A2 (collagen type I alpha 2 chain) were analyzed by western blotting (WB). Relative expression of circ-PRDM5 , miR-92b-3p , and COL1A2 mRNA was analyzed by qRT-PCR. The targeting relationship was confirmed by dual-luciferase reporter and RIP assays. We observed that circ-PRDM5 and COL1A2 were upregulated in PCO tissues and TGF-β2-treated LECs, while miR-92b-3p was downregulated. Both circ-PRDM5 and COL1A2 knockdown impaired TGF-β2-induced LEC migration, invasion, and EMT. Also, circ-PRDM5 could adsorb miR-92b-3p to regulate COL1A2 expression. Furthermore, miR-92b-3p inhibitor offset circ-PRDM5 knockdown-mediated influence on migration, invasion, and EMT of LECs under TGF-β2 stimulation. Also, COL1A2 overexpression overturned the repressive influence of miR-92b-3p mimic on TGF-β2-induced LEC migration, invasion, and EMT. In summary, TGF-β2-induced circ-PRDM5 facilitated LEC migration, invasion, and EMT by adsorbing miR-92b-3p and increasing COL1A2 expression, offering new insights into the development of PCO.