Three new lanostane triterpenoids and two new amides from Alternaria sp. with NLRP3 inflammasome inhibitory activity

• Published in: Natural product research Vol. 38; no. 17; pp. 3041 - 3050
• Main Authors: Bi, De-Wen, Feng, Jian, Pang, Wen-Hui, Yang, Peng-Yun, Xu, Yao-Jun, Aurang Zeb, Muhammad, Wang, Meng-Ru, Zhang, Xing-Jie, Li, Xiao-Li, Zhang, Rui-Han, Wang, Wei-Guang, Xiao, Wei-Lie
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.09.2024; Taylor & Francis Ltd
• Subjects: Acetic acid; Alternaria; Alternaria - chemistry; Alternaria sp. gx-2; Amides; Amides - chemistry; Amides - isolation & purification; Amides - pharmacology; Animals; anti-inflammatory activity; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Endophytes; Ethyl acetate; fungi; Humans; Inflammasomes; Inflammasomes - antagonists & inhibitors; Inflammation; L-Lactate dehydrogenase; Lactate dehydrogenase; Lanosterol - analogs & derivatives; Lanosterol - chemistry; Lanosterol - pharmacology; Magnetic Resonance Spectroscopy; Mice; Molecular Structure; Monophenol Monooxygenase - antagonists & inhibitors; NLR Family, Pyrin Domain-Containing 3 Protein - antagonists & inhibitors; NLRP3 inflammasome; NMR; Nuclear magnetic resonance; spectral analysis; Triterpenes - chemistry; Triterpenes - isolation & purification; Triterpenes - pharmacology; Triterpenoids; Tyrosinase; amides; NLRP3 inflammasome; tyrosinase; Alternaria sp. gx-2; triterpenoids
• ISSN: 1478-6419; 1478-6427; 1478-6427
• DOI: 10.1080/14786419.2023.2211215

Three new lanostane triterpenoids (1-3) along with two new amides fatty compounds (4-5) were isolated from the ethyl acetate extract of a culture of the endophytic fungus Alternaria sp. gx-2. Their structures were identified by 1D and 2D NMR spectral data and HRESIMS. Compounds 1-12 were evaluated for their anti-inflammatory and tyrosinase inhibition activities. The isolated compounds did not show inhibitory activities at a concentration of 100 μM against tyrosinase, while under the concentration of 10 μM, the release of lactate dehydrogenase (LDH) inhibition rate of compound 1 was 54.45%, indicating that compound 1 had moderate anti-inflammatory activity on the activation of NLRP3 inflammasome.