Ac4C modification of lncRNA SIMALR promotes nasopharyngeal carcinoma progression through activating eEF1A2 to facilitate ITGB4/ITGA6 translation

• Published in: Oncogene Vol. 43; no. 38; pp. 2868 - 2884
• Main Authors: Gong, Sha, Qiao, Han, Wang, Jing-Yun, Huang, Sheng-Yan, He, Shi-Wei, Zhao, Yin, Tan, Xi-Rong, Ye, Ming-Liang, Li, Jun-Yan, Liang, Ye-Lin, Huang, Sai-Wei, Chen, Jun, Zhu, Xun-Hua, Liu, Na, Li, Ying-Qing
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 16.09.2024; Nature Publishing Group
• Subjects: 14/32; 38/47; 45/77; 45/90; 631/208/177; 631/337/574; 631/67/1536; 64/60; 692/53; 692/53/2422; 82/51; 82/58; 82/83; Acetyltransferase; Apoptosis; Cancer; Cell activation; Cell adhesion & migration; Cell Biology; Cell growth; Cell proliferation; Human Genetics; Internal Medicine; Localization; Macrophages; Medical prognosis; Medicine; Medicine & Public Health; Metastases; Metastasis; N-Acetyltransferase; Nasopharyngeal carcinoma; Non-coding RNA; Oncology; Phenotypes; Phosphorylation; Protein biosynthesis; Protein synthesis; Proteins; Survival analysis; Throat cancer; Translation elongation; Tumors
• ISSN: 0950-9232; 1476-5594; 1476-5594
• DOI: 10.1038/s41388-024-03133-x

The dysregulation of long non-coding RNAs (lncRNAs) are involved in regulating tumor progression in multiple manner. However, little is known about whether lncRNA is involved in the translation regulation of proteins. Here, we identified that the suppressor of inflammatory macrophage apoptosis lncRNA ( SIMALR ) was highly expressed in nasopharyngeal carcinoma (NPC) tissues by analyzing the lncRNA microarray. Clinically, the high expression of SIMALR served as an independent predictor for inferior prognosis in NPC patients. SIMALR functioned as an oncogenic lncRNA that promoted the proliferation and metastasis of NPC cells in vitro and in vivo. Mechanistically, SIMALR served as a critical accelerator of protein synthesis by binding to eEF1A2 (eukaryotic translation elongation factor 1 alpha 2), one of the most crucial regulators in the translation machinery of the eukaryotic cells, and enhancing its endogenous GTPase activity. Furthermore, SIMALR mediated the activation of eEF1A2 phosphorylation to accelerate the translation of ITGB4 / ITGA6 , ultimately promoting the malignant phenotype of NPC cells. In addition, N-acetyltransferase 10 (NAT10) enhanced the stability of SIMALR and caused its overexpression in NPC through the N4-acetylcytidine (ac4C) modification. In sum, our results illustrate SIMALR functions as an accelerator for protein translation and highlight the oncogenic role of NAT10- SIMALR -eEF1A2-ITGB4/6 axis in NPC.