Effects of a polysaccharide extract from Amomum villosum Lour. on gastric mucosal injury and its potential underlying mechanism

AVLP-2, a novel acidic polysaccharide, was isolated and purified from Amomum villosum Lour. The structural characteristics of the polysaccharides were characterized using monosaccharide composition, methylation, FT-IR, and NMR techniques. Results showed that AVLP-2 comprises galactose and glucose mo...

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Published inCarbohydrate polymers Vol. 294; p. 119822
Main Authors Liu, Huifan, Zhuang, Shanshan, Liang, Churong, He, Junjun, Brennan, Charles Stephen, Brennan, Margert Anne, Ma, Lukai, Xiao, Gengsheng, Chen, Hao, Wan, Shuo
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 15.10.2022
Subjects
Alcohol-induced
Amomum villosum Lour. polysaccharide
animals
galactose
gastric mucosa
Gastric mucosal injure
glucose
inflammation
interleukin-10
malondialdehyde
Mechanism
methylation
molecular weight
oxidative stress
polysaccharides
protective effect
Structure characterization
Wurfbainia villosa
Gastric mucosal injure
Amomum villosum Lour. polysaccharide
Structure characterization
Alcohol-induced
Mechanism
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Summary:AVLP-2, a novel acidic polysaccharide, was isolated and purified from Amomum villosum Lour. The structural characteristics of the polysaccharides were characterized using monosaccharide composition, methylation, FT-IR, and NMR techniques. Results showed that AVLP-2 comprises galactose and glucose monomers, has a molecular weight of 10.488 kDa, and has backbone structures →4)-α-d-GalAp-(1→3,4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→6)-α-d-Galp-(1→, and n→6)-α-d-Galp-(1→4)-β-d-Glcp-(1→, with α-d-Galp-(1→ branches. Furthermore, AVLP-2 had a significant protective effect against oxidative stress in alcohol-induced injury and LPS inflammation models GES-1 cells by regulating the levels of ROS and inflammatory factors. In the animal experiments, AVLP-2 improved the oxidative stress status of the gastric mucosa by increasing SOD activity and GSH levels and inhibiting the excessive generation of malondialdehyde in tissues. The levels of MPO, IL-1β, IL-10, and NF-κB p65 were downregulated, while that of TNF-α was upregulated by AVLP-2 treatment, thereby reducing the alcohol-induced inflammation. [Display omitted]
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ISSN:0144-8617
1879-1344
1879-1344
DOI:10.1016/j.carbpol.2022.119822