First complete genome sequence of an isolate of cowpea severe mosaic virus from South America

• Published in: Virus genes Vol. 57; no. 2; pp. 238 - 241
• Main Authors: Teixeira, Kelvin Josemar M. L., de Souza Cascardo, Renan, Leal, Lorhan L., Zerbini, F. Murilo, Beserra, José Evando A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2021; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Genomes; Medical Microbiology; Next-generation sequencing; Nucleotide sequence; Open reading frames; Plant extracts; Plant Sciences; Poly(A); Polyadenylation; Polyproteins; Ribonucleic acid; RNA; Short Report; Vigna unguiculata; Virology; Brazil; High-throughput sequencing; CPSMV; Comovirus; Secoviridae; Vigna unguiculata
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/s11262-021-01831-2

In this study, the complete nucleotide sequence of a Brazilian isolate of cowpea severe mosaic virus (CPSMV) is presented for the first time. To date, the CPSMV-DG isolate, from the USA, is the only one with the complete known genome. High-throughput sequencing (Illumina HiSeq) and Sanger sequencing of the total RNA extract from a cowpea plant collected in Teresina city, Brazil, revealed the genome sequence of the CPSMV-Ter1 isolate. RNA-1 and RNA-2 are, respectively, 5921 and 3465 nucleotides (nt) long without the poly(A) tail, and show 77.91% and 76.08% nt sequence identity with CPSMV-DG, considered the type isolate of the species. The open reading frames (ORFs) were determined and the cleavage sites of the polyproteins were predicted. Although the two isolates show a similar genomic organization, there was a low percentage of sequence identity between Ter1 and DG. Furthermore, pairwise comparisons of a partial RNA-1 fragment between CPSMV-Ter1 and 11 CPSMV isolates from Brazil indicated 94.6 to 94.8% nt and 98.9% to 99.4% aa sequence identities.