Overexpression of flowering locus D (FLD) in Indian mustard (Brassica juncea) enhances tolerance to Alternaria brassicae and Sclerotinia sclerotiorum

• Published in: Plant cell reports Vol. 42; no. 7; pp. 1233 - 1250
• Main Authors: Shekhar, Shashi, Panwar, Ruby, Prasad, Subhash Chandra, Kumar, Deepak, Rustagi, Anjana
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2023; Springer Nature B.V
• Subjects: Alternaria; Alternaria brassicae; Arabidopsis - genetics; Biomedical and Life Sciences; Biotechnology; Brassica; Brassica juncea; Cell Biology; Crop yield; Environmental effects; Flowering; Fungi; Fungicides; Jasmonic acid; Life Sciences; Loci; Mustard; Mustard Plant - genetics; Mustard Plant - microbiology; Original Article; Pathogens; Physiochemistry; Plant Biochemistry; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Sciences; Reactive Oxygen Species; Redox potential; Sclerotinia sclerotiorum; Transgenes; Systemic acquired resistance; Leaf blight; Stem rot; Flowering locus D (FLD); Reactive oxygen species (ROS); Brassica juncea
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-023-03021-w

Key message Overexpression of BjFLD in Brassica juncea imparts resistance against fungal pathogens and increases the yield. These transgenics could lower the use of fungicides, which have detrimental effects on the environment. Productivity of Indian mustard ( Brassica juncea) is adversely affected by fungal phytopathogens, Alternaria brassicae and Sclerotinia sclerotiorum. Arabidopsis flowering locus D (FLD) positively regulates jasmonic acid signaling and defense against necrotrophic pathogens. In this study, the endogenous FLD ( B. juncea FLD; BjFLD) in Indian mustard was overexpressed in B. juncea to determine its role in biotic stress tolerance. We report the isolation, characterization, and functional validation of BjFLD. The transgene expression was confirmed by qRT-PCR. The constitutive overexpression of BjFLD enhanced the tolerance of B. juncea to A. brassicae and S. sclerotiorum , which was manifested as delayed appearance of symptom, impeded disease progression, and enhanced percentage of disease protection. The transgenic lines maintained a higher photosynthetic capacity and redox potential under biotic stress and could detoxify reactive oxygen species (ROS) by modulating the antioxidant machinery and physiochemical attributes. The BjFLD -overexpressing lines showed enhanced SA level as well higher NPR1 expression. The overexpression of BjFLD induced early flowering and higher seed yield in the transgenic lines. These findings indicate that overexpression of BjFLD enhances the tolerance of B. juncea to A. brassicae and S. sclerotiorum by induction of systemic acquired resistance and mitigating the damage caused by stress-induced ROS.