The enzymatic susceptibility of cellulose microfibrils of the algal-bacterial type and the cotton-ramie type

• Published in: Carbohydrate research Vol. 305; no. 2; pp. 261 - 269
• Main Authors: Hayashi, Noriko, Sugiyama, Junji, Okano, Takeshi, Ishihara, Mitsuro
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.1997
• Subjects: Cellulose I α; Cellulose, algal-bacterial type; Cellulose, cotton-ramie type: Cellulase; Microfibril; Cellulose, cotton-ramie type: Cellulase; Microfibril; Cellulose, algal-bacterial type; Cellulose I α
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/S0008-6215(97)10032-5

Two types of substrates, the algal-bacterial type (rich in cellulose I α) cellulose and the cotton-ramie type (dominant in cellulose I β) cellulose, were degraded comparatively by Trichoderma viride cellulase. The algal-bacterial type cellulose microfibril was more susceptible than the cotton-ramie type. The residual cellulose microfibrils were observed by TEM and analyzed by FTIR and electron diffraction. It becomes clear that the residual cellulose of the algal-bacterial type cellulose was getting rich in the cellulose I β with the lapse time of cellulase treatment. These results indicate that the cellulose I α in the microfibril of the algal-bacterial type cellulose is hydrolyzed preferentially by the cellulase.