Curcumin protects BV2 cells against lipopolysaccharide-induced injury via adjusting the miR-362-3p/TLR4 axis

• Published in: Molecular biology reports Vol. 47; no. 6; pp. 4199 - 4208
• Main Authors: Xie, Ping, Deng, Ming, Sun, Qinguo, Jiang, Bo, Xu, Hongjie, Liu, Junqi, Zhou, Yan, Ma, Yonggang, Chen, Zhonghui
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2020; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Apoptosis; Binding sites; Biomedical and Life Sciences; Caspase-3; Cell injury; Cell Line; Cell Survival - drug effects; Cell viability; Chromosome 3; Curcumin; Curcumin - metabolism; Curcumin - pharmacology; Cytokines - metabolism; Gene deletion; Histology; Hospitals; IL-1β; Inflammation; Inflammation - metabolism; Interleukin 10; Interleukin 6; Life Sciences; Lipopolysaccharides; Lipopolysaccharides - pharmacology; Microglia - drug effects; Microglia - metabolism; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Molecular biology; Morphology; NF-kappa B - metabolism; NF-κB protein; Original Article; Rats; Reagents; Signal Transduction - drug effects; TLR4 protein; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Traumatic brain injury; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; NF-κb; Curcumin; Spinal cord injury; miR-362-3p; TLR4
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-05543-y

Curcumin was demonstrated to be an active ingredient with anti-inflammatory effects. This research was to investigate the effects of curcumin. We found that curcumin promoted cell viability and suppressed cell apoptosis. Meanwhile, curcumin decreased the level of cleaved caspase-3 and the release of TNF-α, IL-1β, IL-6, but increased IL-10 release in LPS-treated BV2 cells. miR-362-3p expression was upregulated by curcumin, while TLR4 expression was downregulated. Besides, we observed that the cytoprotective effects of curcumin were lost when miR-362-3p was silenced. TLR4 was a direct target gene of miR-362-3p. Moreover, miR-362-3p deletion attenuated the cytoprotective effects of curcumin by regulating TLR4 expression in LPS-induced BV2 cells. Furthermore, curcumin suppressed p-p65 expression via regulating miR-362-3p/TLR4 axis. We discovered that curcumin exhibited protective effects against LPS-triggered cell injury via modulating miR-362-3p/TLR4 axis through NF-κB pathway.