Anaerococcus faecalis sp. nov., Isolated from Swine Faeces

• Published in: Current microbiology Vol. 78; no. 7; pp. 2589 - 2594
• Main Authors: Yu, Seung Yeob, Oh, Byeong Seob, Ryu, Seoung Woo, Kim, Ji-Sun, Lee, Jung-Sook, Park, Seung-Hwan, Kang, Se Won, Lee, Jiyoung, Lee, Mi-Kyung, Choe, Hanna, Jung, Won Yong, Jung, Hyunjung, Hur, Tai-Young, Kim, Hyeun Bum, Kim, Jae-Kyung, Lee, Ju-Hoon, Lee, Ju Huck
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2021; Springer Nature B.V
• Subjects: Anaerococcus; Biomedical and Life Sciences; Biotechnology; Catalase; Deoxyribonucleic acid; DNA; Fatty acids; Feces; Gene sequencing; Genomes; Gram stain; Life Sciences; Microbiology; New species; Nucleotide sequence; Nucleotides; Phylogenetics; Phylogeny; rRNA 16S; Sequence analysis; Sodium chloride; Swine; tRNA
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-021-02497-7

An obligate anaerobic, Gram-stain-positive, non-spore forming, non-motile, catalase and oxidase-negative, coccoid-shaped bacterium designated AGMB00486 T was isolated from swine faeces. The optimal growth of the isolate occurred at pH 8.0 and 37 ℃. Furthermore, the growth was observed in the presence of up to 4% (w/v) NaCl but not at salinity levels higher than 5%. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain AGMB00486 T was a member of the genus Anaerococcus and that the isolate was most closely related to Anaerococcus vaginalis KCTC 15028 T (96.7% 16S rRNA gene sequence similarity) followed by Anaerococcus hydrogenalis KCTC 15014 T (96.7%) and Anaerococcus senegalensis KCTC 15435 T (96.3%). Whole-genome sequence analysis determined that the DNA G+C content of strain AGMB00486 T was 30.1 mol%, and the genome size, numbers of tRNA and rRNA genes were 2,268,866 bp, 47 and 8, respectively. The average nucleotide identity values between strain AGMB00486 T and the three related type strains were 77.0, 77.4 and 77.2%, respectively. The major cellular fatty acids (> 10%) of strain AGMB00486 T were C 14:0 , C 16:0 and C 16:0 DMA. Accordingly, these distinct phenotypic and phylogenetic properties revealed that strain AGMB00486 T represents a novel species, for which the name Anaerococcus faecalis sp. nov. is proposed. The type strain is AGMB00486 T (= KCTC 15945 T  = CCTCC AB 202009 T ).