Intracellular activity of pharyngeal motoneurons during breathing, swallowing, and coughing

• Published in: Journal of neurophysiology Vol. 124; no. 3; pp. 750 - 762
• Main Authors: Umezaki, Toshiro, Shiba, Keisuke, Sugiyama, Yoichiro
• Format: Journal Article
• Language: English
• Published: 01.09.2020
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00093.2020

We have provided the first demonstration of the multifunctional activity of the pharyngeal motoneurons at the level of membrane potential during respiration, swallowing, and coughing. We recorded membrane potentialp changes in 45 pharyngeal motoneurons (PMs) including 33 expiratory modulated and 12 nonrespiratory neurons during breathing, swallowing, and coughing in decerebrate paralyzed cats. Four types of membrane potential changes were observed during swallowing: 1) depolarization during swallowing ( n = 27), 2) depolarization preceded by a brief (≤ 0.1 s) hyperpolarization ( n = 4), 3) longer term (> 0.3 s) hyperpolarization followed by depolarization ( n = 11), and 4) hyperpolarization during the latter period of swallowing ( n = 3). During coughing, PMs showed two types of membrane potential changes ( n = 10). Nine neurons exhibited a ramp-like depolarization during the expiratory phase of coughing with the potential peak at the end of expiratory phase. This depolarization was interrupted by a transient repolarization just before the potential peak. The membrane potential of the remaining neuron abruptly depolarized at the onset of the expiratory phase and then gradually decreased even after the end of the expiratory phase. Single-shock stimulation of the superior laryngeal nerve (SLN) induced inhibitory postsynaptic potentials in 19 of 21 PMs. Two motoneurons exhibited an SLN-induced excitatory postsynaptic potential. The present study revealed that PMs receive the central drive, consisting of a combination of excitation and inhibition, from the pattern generator circuitry of breathing, swallowing, and coughing, which changes the properties of their membrane potential to generate these motor behaviors of the pharynx. Our data will provide the basis of studies of pharyngeal activity and its control from the medullary neuronal circuitry responsible for the upper airway motor activity. NEW & NOTEWORTHY We have provided the first demonstration of the multifunctional activity of the pharyngeal motoneurons at the level of membrane potential during respiration, swallowing, and coughing.